汉族人群血小板同种抗原HPA-3、HPA-9w多态性分布

目的研究我国不同汉族人群血小板同地区种抗原HPA-3、HPA-9w多态性。方法采用聚合酶链反应-序列特异性引物(polymerase chain reaction-sequence specific primers,PCR-SSP)技术对1000名来自不同省份汉族无关献血者进行HPA-3、HPA-9w抗原基因分型。结果在调查的1000名汉族人群中HPA-3a、HPA-3b基因频率分别为0.5935和0.4065,HPA-9全为a/a纯合子。经χ2检测,符合Hardy-Weinberg平衡。不同地区汉族人群之间比较,广东省与陕西、黑龙江、浙江、云南、江苏5省的HPA-3多态性分布差异有统计学意义,其余省份之间多态性分布差异无统计学意义。中国汉族人群与越南人、澳大利亚人HPA-3的多态性分布差异有统计学意义。结论在随机输血中供受者HPA-3抗原不配合比例达0.3661,这为研究同种免疫血小板减少症、开展血小板同型输注提供了理论基础。

Polymorphism of the human platelet alloantigens HPA-3 and HPA-9w in the Chinese Han population

Objective To study the polymorphism of human platelet alloantigens HPA-3 and HPA-9w in the Chinese Han population.Methods A total of 1000 unrelated Chinese Han blood donors from different provinces of China were genotyped for HPA-3 and HPA-9w using PCR-sequence specific primer assay.Results Gene frequencies of 1000 Chinese Hans for HPA-3a and HPA-3b were 0.5935 and 0.4065 respectively,and all of them were HPA-9a positive.The distributions of HPA-3,HPA-9w of Chinese Hans which detected by chi-square criterion fit Hardy-Weinberg equilibrium.There were significant differences of the HPA-3 alleles gene frequency between Guangdong province and other five investigated provinces which included Shanxi,Heilongjiang,Zhejiang,Yunnan and Jiangsu.In comparison to other ethnic groups,no significant differences were observed in the distributions of HPA-3 except the Vietnamese and Australian.Conclusion The results show that the chance of HPA-3 incompatibility were 0.3661 in random transfusion,and also provide a basis for researching on alloimmune thrombocytopenia and HPA-matched transfusion.