早期生长反应因子-1及乙酰肝素霉在膀胱移行细胞癌中的表达及意义

目的：探讨早期生长反应蛋白Egr-1在膀胱移行细胞癌中的表达和意义以及与乙酰肝素酶heparanase的相关性。方法：采用免疫组织化学SABC（StreptAvidin-Biotin Complex）法对58例膀胱移行细胞癌组织及10例正常膀胱粘膜组织进行Egr-1、heparanase的检测，并对两者表达情况与BTCC临床病理特征的关系，两者在BTCC中表达的相关性进行了统计分析。结果：Egr-1蛋白在10例正常膀胱组织中不表达，而58例BTCC组织中阳性表达占70.69%(41/58)，差异有显著意义(P<0.01); heparanase蛋白在10例正常膀胱组织中不表达，而在58例BTCC组织中阳性表达占58.62%(34/58)，差异有显著意义(P<0.01); Egr-1及heparanase阳性表达率在不同年龄、性别、肿瘤大小及数目的患者之间，无显著性差异（P>0.05);随着病理分级及临床分期增加，Egr-1及heparanase蛋白表达率及表达强度均增加。在BTCC中Egr-1蛋白与heparanase表达呈正相关(r =0.3875,P<0?01)。结论: Egr-1及heparanase蛋白的高表达在膀胱移形细胞癌的发生、发展中起重要作用，与非肌层浸润型膀胱癌进展为肌层浸润型膀胱癌过程相关。Egr-1可作为判断膀胱移形细胞癌生物学行为的重要指标,并且对临床开展肿瘤防治新途径提供理论依据。

Expression of Egr-1 protein and heparanase in bladder transitional cell carcinoma and its clinical significance

Objective: To investigate the relationship between the expression of Egr-1 protein andheparanase.Methods: The expression of Egr-1and heparanase was examined by SABC（StreptAvidin-Biotin Complex）immunohistochemistry in 58 Paraffin-embedded tissue specimens and 10 control groups of normal bladder tissues.The expression and relationship of Egr-1 and heparanase with clinicopathologic factors and the relationship of Egr-1 and heparanase were analysed . Results: The positive expression rate of Egr-1 in normal bladder tissue was 0%(0/10)，while the positive expression rate of Egr-1 in BTCC was statistically significant(P<0.01).No heparanase expression was detectable in 10 normal bladder tissues，while heparanase expression was detected in 34 of 58 transitional cell carcinoma of bladder(58.62%)。The positive expression of Egr-1 and heparanase in transitional cell carcinoma of bladder had no distinct difference in age，sex，tumorsize and tumor quantity(P>0.05)，but it had a close correlation with the degrees of histological grades and clinical stages（P<0.01). The expression of Egr-1 was closely correlated with that of heparanase (liner index of Pearson=0.3875，P<0.01). Conclusion: the positive experssion of Egr-1 and heparanase might be the referencing indxe of the development and invasion of bladder carcinoma and support a potential involvement of Egr-1 in the progression from non-muscle invasive bladder cancers to muscle invasive bladder cancer. It is shown that Egr-1 may provide theoretical fundation of chemopreventive stategy for bladder cancer in the future.