亚低温对颅脑损伤患者神经元特异性烯醇化酶和S-100蛋白含量的影响

目的研究亚低温治疗对颅脑损伤患者血清神经元特异性烯醇化酶（NSE）和S-100的影响及临床意义。方法中重型颅脑损伤患者57例,分为亚低温组和常温组,亚低温组与伤后24h内行亚低温治疗,控制直肠温度33~35℃,持续3~5d,各组患者分别于伤后1d,3d,部分患者5d和7d外周静脉采血标本,另以健康者20例作对照组;采用酶联免疫吸附法测定血清中含量。结果颅脑损伤患者血清中NSE和S-100含量分别为（13.87±2.57）mg/L和（0.34±0.12）μg/L,与正常组（6.51±2.31）mg/L和（0.17±0.07）μg/L相比明显增高,持续3~5d,伤后明显下降,但仍高于正常;亚低温组治疗期间及复温后血清中含量低于常温组（P〈0.01）。结论颅脑损伤患者血清S-100和NSE升高,亚低温治疗能降低它们的含量,改善患者的预后。

EFFECT OF MILD HYPOTHERMIA THERAPY ON NSE AND S-100 IN SERUM AFTER TRAUMATIC BRAIN INJURY

Objective To study the effect and clinical significance of mild hypothermia therapy on NSE and S-100 in serum after traumatic brain injury.Methods 57 patients with mid or severe injured traumatic brain injury were divided into two groups randomly.29 cases of hypothermia group were treated with mild hypothermia of 33~35℃ for 3~5 days after injury,while 28 cases of the normothermic group were maintained at normothermic levels（36~37℃）.S-100 and NSE levels were measured at 1,3,5,7d after injury in serum by using the enzyme linked immunosorbent assay.Other 20 healthy people were selected to determine normal ranges.Results The levels of serum S-100 and NSE in the injured patients were（13.87±2.57）mg/L and（0.34±0.12）μg/L respectively,which significantly higher than that of healthy people（（6.51±2.31）mg/L,（0.17±0.07）μg/L）and which last 3~5d,lower after injury but still higher than normal.In the hypothermia group,the serum S-100 and NSE levels were significantly lower than the normothermic group（P0.01）.Conclusion Serum S-100 and NSE levels are increased after traumatic brain injury;and mild hypothermia therapy can decrease them and improve the prognosis of the patients.