大鼠椎骨生物力学与骨组织形态计量学的灰色关联分析

目的:运用灰色关联分析,探讨大鼠椎骨生物力学与骨组织形态计量学之间的相关关系.方法:10.5月龄未交配的雌性SD大鼠48只,随机分为6组:① 10.5月龄基础对照组(Basal);② 13月龄假手术组(Sham-1);③ 13月龄去卵巢10周组(OVX-1);④ 16月龄假手术组(Sham-2);⑤ 16月龄去卵巢22...

Analysis of Grey Correlation between Bone Bio-mechanical Property and Histomorphometry of Lumbar Vertebrae Body in Rats

Objective： To study the correlative changes of bone bio-mechanical property and histomorphometry of lumbar vertebrae in rats by analysis of grey correlation.Methods： Forty-eight 10.5-month-old virgin female Sprague-Dawley rats were randomly divided into six groups： ① Basal： at 10.5 month of age; ② sham-1： sham-operated at 13 month of age;③ OVX-1： ovariectomized at 13 month of age;④ sham-2： sham-operated at 16 month of age;⑤ OVX-2： ovariectomized at 16 month of age;⑥ OVX-2＋EE： ovariectomized and treated with 17a-ethynylestradiol（100μg·kg-1· d-1） starting at 13 month of age for 12 weeks.At the end-point of study,the undecalcified longitudinal fourth lumbar vertebra（LV4） sections were cut for bone histomorphometric analyses.The mechanical properties of the fifth lumbar vertebra body（LV5） were measured with compression test.Then the effects of bone histomorphometry on bio-mechanical property were assessed by analyzing the T-type correlation degree in the Grey system.Results： All degrees of correlation between bone bio-mechanical property（break load,break stess and elastic modulus） and histomorphometric parameters（percent trabecular area（%Tb.Ar）,trabecular thickness（Tb.Th））were positive,but for trabecular sepration（Tb.Sp）,bone turnover rate（BFR/BV）,the correlation degrees were negative.The effect of %Tb.Ar and Tb.Th on bio-mechanical property of lumbar vertebrae was much greater than that of the other histomorphometric parameters.Conclusions： Analysis of grey correlation could evaluate the correlative changes of bone bio-mechanical property and histomorphometry of lumbar vertebrae in rats objectively and fairly.