番茄红素诱导人结肠癌SW480细胞凋亡的作用机制

 目的
探讨番茄红素诱导人结肠癌SW480细胞凋亡的分子机制。方法体外培养SW480细胞,采用MTT比色实验、流式细胞术、RT-PCR和Western
blot方法,分析番茄红素对SW480细胞的生长增殖作用、对细胞凋亡率的影响及对细胞凋亡相关分子Bcl-2、Bax、Caspase-9和
Caspase-3表达的影响。结果MTT法显示,番茄红素能显著抑制SW480细胞的生长增殖能力,且呈一定的量效与时效关系,24 h的半数抑
制浓度(IC50)为1.0 μmol/L；流式细胞仪分析表明,番茄红素呈浓度依赖性诱导SW480细胞凋亡；RT-PCR或Western blot分析表
明,SW480细胞经不同浓度番茄红素诱导后,与溶媒组相比,Bcl-2表达逐渐减弱,而Bax,Caspase-9和Caspase-3表达逐渐增强,Bcl-
2/Bax比值降低。结论番茄红素对SW480细胞的抑制增殖作用,可能与降低Bcl-2/Bax比值、增加Caspase-9和Caspase-3蛋白表达、诱
导细胞凋亡有关。

Mechanism of Lycopene Induced Cell Apoptosis on Human Colon Cancer SW480 Cells

Objective
To investigate the effect of Lycopene on the apoptosis of human colon cancer SW480 cells and the relevant molecular
mechanism.MethodsThe anti-proliferative effect of a different concentration of Lycopene on SW480 cells was measured
by MTT assay.Ratio of SW480 cells apoptosis was analyzed by flow cytometry.The levels of Bcl-2,Bax were detected by
RT-PCR and Western blot.The expression levels of Caspase-9 and Caspase-3 were analyzed by Western blot.ResultsThe
MTT assay showed that Lycopene had potent anti-proliferative effect on SW480 cells in a time- and dose-dependent
manner.The IC50 value of Lycopene was SW480 cells treated with 10 μmol/L Lycopene for 24 hours.Flow cytometry
analysis revealed that Lycopene increased the percentage of apoptotic SW480 cells.The expression level of Bcl-2
were decreased,while the expression levels of Bax,Caspase-9 and Caspase-3 were increased significantly in SW480
cells treated with different concentrations of Lycopene.ConclusionLycopene could induce apoptosis of SW480 cells by
decreasing the ratio of Bcl-2/Bax and increasing Caspase-9 and Caspase-3 expression.