| UPLC-MS/MS测定青蒿素代谢抑制剂猫眼草黄素及大鼠血浆药代动力学研究 |
| |
| 引用本文： | 陈靖,纪红燕,魏世杰,付雪艳,刘梦英,吴秀丽.UPLC-MS/MS测定青蒿素代谢抑制剂猫眼草黄素及大鼠血浆药代动力学研究[J].中国中药杂志,2013,38(19):3363-3367. |
| |
| 作者姓名： | 陈靖 纪红燕 魏世杰 付雪艳 刘梦英 吴秀丽 |
| |
| 作者单位： | 宁夏医科大学 药学院, 宁夏 银川 750004;宁夏医科大学 药学院, 宁夏 银川 750004;宁夏医科大学 总医院 临床药理研究室, 宁夏 银川 750004;宁夏医科大学 药学院, 宁夏 银川 750004;宁夏医科大学 药学院, 宁夏 银川 750004;宁夏医科大学 药学院, 宁夏 银川 750004 |
| |
| 基金项目： | 国家自然科学基金项目(30960465) |
| |
| 摘 要： | 目的: 建立UPLC-MS/MS测定大鼠血浆中青蒿素代谢抑制剂猫眼草黄素(chrysosplenetin,CHR)的体内分析方法,并考察其药代动力学参数. 方法: 血浆样品以乙腈沉淀蛋白;采用Shim-pack XR-ODS C18 色谱柱(2.0 mm ×100 mm,2.2 μm),以地西泮为内标,流动相为甲醇-0.1%甲酸水溶液(87:13);质谱条件为电喷雾离子源(ESI源),正离子模式检测;样品分析时间为2 min. 结果: CHR在5~5 000 μg·L-1线性良好(r=0.999 3),提取回收率为69.0%~81.2%,日内、日间精密度和准确度符合要求.CHR口服吸收差,存在双峰甚至多峰现象;静脉注射消除半衰期短,低、中、高剂量组t1/2分别为(17.01±8.06),(24.62±4.59),(28.46±4.63) min. 结论: 该方法特异、快速、灵敏,可用于CHR药代动力学分析.
|
| 关 键 词： | UPLC-MS/MS 猫眼草黄素 药代动力学 血浆 大鼠 |
| 收稿时间： | 4/4/2013 12:00:00 AM |
Determination of chrysosplenetin, metabolic inhibitor of artemisinin, in rat plasma by UPLC-MS/MS and study on its pharmacokinetics |
| |
| CHEN Jing,JI Hong-yan,WEI Shi-jie,FU Xue-yan,LIU Meng-ying and WU Xiu-li.Determination of chrysosplenetin, metabolic inhibitor of artemisinin, in rat plasma by UPLC-MS/MS and study on its pharmacokinetics[J].China Journal of Chinese Materia Medica,2013,38(19):3363-3367. |
| |
| Authors: | CHEN Jing JI Hong-yan WEI Shi-jie FU Xue-yan LIU Meng-ying and WU Xiu-li |
| |
| Institution: | School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China;School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China;Institute of Clinical Pharmacology, General Hospital of Ningxia Medical University, Yinchuan 750004, China;School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China;School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China;School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China |
| |
| Abstract: | |
| |
| Keywords: | UPLC-MS/MS chrysosplenetin pharmacokinetics plasma rat |
|
|
