反相高效液相色谱法同时测定芪香胶囊中红景天苷、芍药苷及毛蕊异黄酮葡萄糖苷的含量

目的：建立高效液相色谱法同时测定芪香胶囊（黄芪、红景天、赤芍、红花）中红景天苷、芍药苷、毛蕊异黄酮葡萄糖苷3种活性成分的含量。方法：采用Phenomenex Luna色谱柱（250 mm×4.6 mm，5 μm），以乙腈（A）和水（B）为流动相，梯度洗脱[0~11 min，A-B（10：90），11~40 min，A-B（18：82），40~45 min，A-B（10：90）]，流速1.0 mL·min^-1，柱温为35℃，检测波长：230 nm。结果：红景天苷在55.07~192.8 μg·mL^-1、芍药苷在65.45~229.1 μg·mL^-1、毛蕊异黄酮葡萄糖苷在10.74~64.44 μg·mL^-1 范围内均呈良好线性关系；相关系数分别为0.999 8，0.999 7，0.999 8；红景天苷、芍药苷和毛蕊异黄酮葡萄糖苷的回收率分别为99.90%，100.88%，100.06%，样品溶液在24 h内稳定，含量分别为1.422 2，3.183 3，0.250 0 mg·g^-1。结论：该方法经验证，可为芪香胶囊多指标质量评价及控制提供科学依据。

Simultaneous determination of salidroside,paeoniflorin and calycosin-7-O-β-D-glucoside in Qixiang Capsules by HPLC

OBJECTIVE To establish an HPLC method for simultaneous determination of salidroside, paeoniflorin and calycosin-7-O-β-D-glucoside in Qixiang Capsules (Astragalus membranaceus, Rhodiola hobsonii, Paeonia veitchii Lynch, Cdrthamus tinctorius L.).METHODS The determination was carried out over Phenomenex Luna column (250 mm×4.6 mm, 5μm) at the column temperature (35℃). The mobile phase consisted of acetonitrile (A)-water (B) solution with the gradient elution[0-11 min, A-B(10:90), 11-40 min, A-B(18:82), 40-45 min, A-B(10:90)], the flow rate was 1.0 mL min^-1 and detection wavelength was 230 nm.RESULTS The method had good linear relationship within the range of 55.07-192.8 μg·mL^-1 for salidroside (r=0.999 8), 65.45-229.1 μg·mL^-1 for paeoniflorin (r=0.999 7) and 10.74-64.44 μg·mL^-1 for calycosin-7-O-β-D-glucoside (r=0.999 8). The average recovery was 99.90% for salidroside, 100.88% for paeoniflorin and 100.06% for calycosin-7-O-β-D-glucoside. The sample solution was stable within 24 h. Contents were 1.422 2, 3.183 3 and 0.250 0 mg·g^-1.CONCLUSION The method can be used to provide scientific evidence for the comprehensive quality evaluation and control of Qixiang Capsule through the methodological validation.