ATP生物荧光法在医疗器械清洗质量检测中的应用

目的 提高器械清洗效果,控制医院感染的危险环节。方法 采用(ATP)生物荧光法对医疗器械清洗效果进行检测,对不同清洗方法及器械类型的清洗效果进行比较。结果 供应室的器械清洗合格率为78.37%,比手术室的合格率66.90%高(P<0.05);经线性趋势检验得:供应室与手术室的器械清洗合格率均逐年上升;机器清洗合格率为75.27%,比手工清洗的合格率51.48%高(P<0.05);手工+机器清洗合格率为87.50%,比手工清洗和机器清洗高(P<0.05)。有齿类和无齿类器械的清洗合格率分别为85.31%、84.82%,2者之间无差异;管腔类器械的清洗合格率为49.55%,比有齿类和无齿类器械低,清洗效果较差(P<0.05)。结论 ATP生物荧光法可以快速评价医疗器械清洗效果,发现医院感染的危险环节。

Application of ATP bioluminescence assay in detecting cleaning quality of medical devices

Objective To improve the effect of instrument cleaning and control the risk factors. Methods ATP bioluminescence assay was used for detecting the effect of instrument cleaning, and the cleaning effects of different cleaning methods and instrument types were compared. Results The cleaning qualified rate of the supply center (78.37%) was higher than that of the clinical departments (66.90%, P<0.05).The cleaning qualified rate of the supply center and clinical departments were increasing year by year. The cleaning qualified rate of machine cleaning (75.27%) was higher than that of manual cleaning (51.48%, P<0.05), and the cleaning effect of manual plus machine cleaning (87.50%) was better than that of the manual cleaning and machine cleaning (P<0.05). The cleaning effect of lumen instrument (49.55%) was worse than those of the toothed instrument (85.31%) and toothless instrument (84.82%, P<0.05). Conclusion ATP bioluminescence assay can rapidly evaluate the effect of instrument cleaning and find the risk factors of nosocomial infection.