超高效液相色谱法同时测定南、北五味子中的7种木脂素含量

目的：建立UPLC测定五味子中7种木脂素的方法，并测定不同地区的南、北五味子中木脂素成分含量。方法：色谱柱ZORBAX Eclipse Plus C₁₈ column （1.8 μm，100 mm×2.1 mm）；流动相乙腈-水，梯度洗脱；流速0.3 mL·min^-1；柱温40℃；检测波长220 nm；进样体积1 μL。结果：各成分即五味子醇甲、五味子醇乙、五味子酯甲、五味子酯乙、五味子甲素、五味子乙素、五味子丙素之间基本达到基线分离，且线性关系良好，线性范围分别为0.984~246.000，0.636~159.000，0.552~138.000，0.472~1.180，0.952~2.380，1.120~2.810，0.436~1.090 μg·mL^-1（r>0.999 6）。平均加样回收率在98.29%~102.5%之间，RSD在1.4%~1.9%之间。结论：所建立的测定方法快速、稳定、可靠，适用于不同产地南、北五味子中木脂素含量的测定。

Simultaneous determination of seven lignans in Schisandra sphenanthera Rehd.et Wils. and Schisandra Chinensis(Turcz.) Baill. by UPLC

OBJECTIVE To establish an UPLC method for determination of seven lignans, and measure lignan contents in Schisandra sphenanthera Rehd.et Wils. and Schisandra Chinensis (Turcz.) Baill. collected from different origins.METHODS A ZORBAX Eclipse Plus C₁₈ column (1.8 μm, 100 mm×2.1 mm) was at the temperature of 40℃, flow rate at 0.3 mL·min^-1 with a mobile phase consisting of acetonitrile and water gradient elution. The samples were analyzed with a small volume (1 μL) per injection. And the detection wavelength was operated at 220 nm within all analyzing time.RESULTS Almost all the compounds (schisandrol A, schisandrol B, schisantherin A, schisantherin B, schisandrin A, schisandrin B and schisandrin C) achieved baseline separation. Sol A, Sol B, Sth A, Sth B, Sin A, Sin B and Sin C also had favorable linearity in the range of 0.984-246.000,0.636-159.000,0.552-138.000,0.472-1.180,0.952-2.380,1.120-2.810 and 0.436-1.090 g·mL^-1 (r>0.999 6), respectively. This method showed a excellent recovery of 98.29%-102.5% with a RSD of 1.4%-1.9%.CONCLUSION This method is rapid, stable and reliable, which can be used for the determination of lignan contents in Schisandra sphenanthera Rehd. et Wils. and Schisandra Chinensis (Turcz.) Baill. from different habitats.