基于Oncomine和GEPIA数据库分析 NFE2L3基因在结直肠癌中的表达及其临床意义

目的通过挖掘Oncomine和GEPIA数据库中的相关数据，分析NFE2L3基因在结直肠癌中的表达及临床意义。 方法检索Oncomine和GEPIA数据库中有关结直肠癌的数据并结合文献资料进行二次综合分析。癌与正常组织之间基因表达差异采用t检验，采用单因素方差分析比较不同临床分期之间基因表达的差异，采用Pearson相关分析进行相关性研究，采用Kaplan-Meier方法进行生存分析。 结果Oncomine数据库中共收集了334项关于NFE2L3基因在不同类型癌与正常组织中表达的比较的研究结果，表达差异有统计学意义的研究结果有55个。其中共有21项研究涉及NFE2L3基因在结直肠癌组织和正常结直肠组织中的表达，共包括1000个样本。与对照组相比结直肠癌组织中NFE2L3 mRNA表达显著高于结直肠正常组织（P<0.05）。GEPIA数据库中分析发现NFE2L3基因的表达与错配修复基因MLH1、MSH2、MSH6和PMS2的表达均呈显著相关性（P<0.05）；NFE2L3基因与结直肠癌的临床分期与预后生存（总生存率和无病生存率）均不具有相关性（P>0.05）。 结论NFE2L3 mRNA在结直肠癌组织中呈高表达，并与影响结肠癌发生的错配修复基因的表达关系密切。

Clinical significance of expression of NFE2L3 gene in colorectal cancer: An analysis based on data-mining of Oncomine and GEPIA databases

ObjectiveTo obtain the gene expression signatures of NFE2L3 and analyze its clinical significance by mining the data from Oncomine and GEPIA databases. MethodsDifferential NFE2L3 mRNA expression in colorectal cancer (CRC) was detected based on the Oncomine and GEPIA databases. Correlations between MTERF3 mRNA expression and mismatch repair genes, clinical stages and prognosis in CRC were analyzed by secondary analysis of the GEPIA database. Gene expression differences between cancer and normal tissues were analyzed by the t-test. One-way analysis of variance was used to compare gene expression between different clinical stages. Person correlation analysis was used for correlation study, and Kaplan-Meier method was used for survival analysis. ResultsA total of 334 different studies were collected in the Oncomine database, of which 55 showed statistically significant expression of NFE2L3. A total of 21 studies involved the expression of NFE2L3 in colorectal cancer tissues and normal tissues, including a total of 1000 samples. Compared with the control group, the expression of NFE2L3 mRNA in colorectal cancer tissues was significantly higher than that in normal colorectal tissues (P<0.05). In the GEPIA database, the expression of NFE2L3 gene was significantly correlated with the expression of mismatch repair genes MLH1, MSH2, MSH6 and PMS2 (P<0.05). However, there was no correlation between clinical stage and colorectal cancer survival (overall survival and disease-free survival) (P>0.05). ConclusionNFE2L3 mRNA is highly expressed in colorectal cancer tissues and is closely related to the expression of mismatch repair genes.