人牙髓细胞来源外泌体对脐静脉内皮细胞迁移能力的影响

目的探讨牙髓细胞外泌体（DPC-Exos）对脐静脉内皮细胞（HUVEC）迁移能力的影响。 方法超速离心法分离提纯DPC-Exos,透射电镜和蛋白印迹法（Western blot）进行鉴定;划痕实验和Transwell实验检测DPC-Exos对HUVEC迁移的影响。采用SPSS 20.0软件进行分析,划痕实验迁移率和Transwell实验细胞迁移数采用两独立样本的t检验进行统计。 结果DPC-Exos呈双层膜包被和茶托样结构,表达CD63膜蛋白标记物。划痕实验结果显示,DPC-Exos作用6 h后HUVEC细胞迁移能力下降26%,差异具有统计学意义（t= 6.534,P<0.001）。Transwell实验结果显示,DPC-Exos作用6 h后HUVEC迁移能力下降32%,差异具有统计学意义（t= 5.846,P<0.001）。 结论DPC-Exos抑制HUVEC迁移。

The roles of dental pulp cell derived exosomes in human umbilical vein endothelial cells migration

ObjectiveTo investigate the effect of dental pulp cell derived exosomes (DPC-Exos) on human umbilical vein endothelial cells (HUVECs) migration in vitro. MethodsExosomes were isolated from the supernatant of DPCs cell culture by ultracentrifugation and characterized by transmission electron microscope (TEM) , western blotting. The effect of DPC-Exos on HUVECs migration was examined by wounding healing assay and transwell assay in vitro. Two independent samples t test was used to statistically analyze the migration rate and number of migration cells by SPSS 20.0 software. ResultsThe bi-layer membrane and "saucer-like" appearance of DPC-Exos were examined by transmission electron microscopy (TEM) . Western blotting revealed that CD63 was expressed in the DPC-Exos. Wound-healing assay showed significantly reduced migration ability by 26% in HUVECs after treated with DPC-Exos (t= 6.534, P<0.001) . Transwell assay results indicated that DPC-Exos treatment could significantly reduced migration ability of HUVECs by 32% (t= 5.846, P<0.001) . ConclusionDPC-Exos could significantly suppress the migration of HUVECs.