水飞蓟素对单侧输尿管梗阻大鼠肾脏纤维化的影响

目的探讨水飞蓟素对单侧输尿管梗阻（UUO）大鼠肾间质纤维化的作用及其可能机制。方法雄性SD大鼠36只,随机分为3组：假手术组（C组）、模型组（M组）及水飞蓟素治疗组（S组,50mg·kg^-1·d^-1）,每组12只。术后第7、21天分别处死每组大鼠6只,光镜观察梗阻肾组织病理变化,并分别用免疫组织化学方法及RT-PCR方法半定量检测各组肾组织骨桥蛋白（OPN）和纤维连接蛋白（FN）的蛋白质及mRNA表达水平。结果M组大鼠肾小管间质损伤及纤维化明显,S组病变与其相似但显著减轻（P〈0．05）;与C组相比,M组OPN和FN的mRNA及蛋白质表达水平均显著上调（P〈0．01）,S组表达较M组明显下降（P〈0．01,P〈0．05）。结论水飞蓟素能抑制OPN和FN的表达进而减少细胞外基质的沉积而发挥肾脏保护作用。

The effect of silymarin on tubulointerstitial fibrosis after unilateral ureteral obstruction in rats

Objective To investigate the effect of silymarin on tubulointerstitial fibrosis after unilateral ureteral obstraction （UUO） and the possible mechanism. Methods Thirty-six male SD rats were randomly divided into three groups： control group （C, subject to sham operation）, UUO group （M） and UUO group treated with silymarin （S, 50 mg/kg every day）. Half of the rats in each group were sacrificed on the postoperative day 7 and 21. The pathological changes in the renal tissue were examined by HE, PAS and Masson stain. Immunohistochemistry and reverse transcription- polymerase chain reaction （RT-PCR） were applied to detect the expression of OPN and FN mRNA and protein. Results Interstitial fibrosis was observed under the microscopy in UUO groups, but the pathological change was more severe in group M than in group S （P〈0. 05）. Irnmunohistochemistry and RT-PCR revealed that the expression of OPN and FN in UUO groups was increased significantly as compared with group C （P〈0. 01 ）, and that was lower in group S than in group M （P〈0. 01）. Conclusions Silymarin may prevent the development of interstitial fibrosis in UUO rats through inhibiting the expression of OPN and FN.