不同工艺制备的补肾中药对拟痴呆小鼠脑组织中乙酰胆碱酯酶活性和M受体功能的影响

将补肾中药按水煎浓缩、水煎醇沉和乙醇提取三种传统加工工艺制成口服液，观察它们对氯化铝溶液所致拟痴呆小鼠记忆功能的影响。每种药液均以高（10mL／k／日）和低（3．3mL／kg／日）两种剂量灌胃，并以同龄、同种小鼠为对照组。在喂药过程中，每10天用避暗法测定各组每只小鼠记忆功能。连续喂药30天后，断头分离出每只小鼠的大脑皮层组织，用酶底物反应和受伤协郎配某结会分析．测字各组小鼠大脑皮层组织中乙酞胆碱酯酶（A。＊E）活性和胆碱能M受体结合容量（Rt）值。实验结果表明，拟痴呆小鼠大脑皮层组织中A。hE活性较对照组明显升高（P＜0．05），M受体Rt值显著下降（P＜0．05）。三种不同加工工艺药液的高低两种剂量都能明显降低A。hE活性，升高M受体Rt值，与同组治疗前比较，差异显著（P＜0．05—0．001）。与小鼠整体迷宫实验能显著提高记忆力相一致。各组间比较差异不明显。证明三种不同制药工艺都可以提取补肾中药的有效成分。水煎浓缩方法简便，价格便宜，应为首选方法。

Effect of Variously Processed Kidney-benefiting Chinese Herbal Medicine on Acetylcholinesterase Activity and M-cholinergic Receptor in the Brain of Demerntia Mice

Kidney-benefiting Chinese herbal medicine (KBCHM) was processed by three traditional ways and was given to dementia mice induced with aluminum chloride to observe its effect on acetylcholinesterase activity (AchE) and M cholinergic receptor Rt in mice cerebral cortex and their memory. AchE activity was significantly increased (P < 0. 05)and M cholinergic receptor Rt was markedly lowered (P < 0. 05) in dementia mice than in the controls. All KBCHM from three traditional processes reduced AchE activity and increased M receptor Rt, which weresignificantly different from those before treatment (P < 0. 05-0. 001 ) and showed no differenceamong three kinds of KBCHM. The results were also consistent with improved memory of miceduring maze test. It is concluded that the effective components of KBCHM can be extracted by allthree processes, among which the decocted and enriched KBCHM is of first choice due to convinient processing and low price.