Engineered NS1 for Sensitive,Specific Zika Virus Diagnosis from Patient Serology |
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| Authors: | Thai Leong Yap Shin Yee Hong Jun Hui Soh Lekha Ravichandraprabhu Vanessa W.X. Lim Hsi-Min Chan Tommy Z.X. Ong Ying Ping Chua Shi En Koh Huajing Wang Yee Sin Leo Jackie Y. Ying William Sun |
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| Affiliation: | Experimental Drug Development Centre, Singapore (T.L. Yap, S.Y. Hong);Institute of Bioengineering and Nanotechnology, Singapore (T.L. Yap, S.Y. Hong, L. Ravichandraprabhu, T.Z.X. Ong, Y.P. Chua, S.E. Koh, H. Wang, W. Sun);NanoBio Lab, Singapore (J.H. Soh, H.-M. Chan, J.Y. Ying);National Centre for Infectious Diseases, Singapore (V.W.X. Lim, Y.S. Leo);Tan Tock Seng Hospital, Singapore (Y.S. Leo);Yong Loo Lin School of Medicine, National University of Singapore (Y.S. Leo, W. Sun). |
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| Abstract: | Dengue virus (DENV) and Zika virus (ZIKV) belong to the Flaviviridae family of viruses spread by Aedes aegypti mosquitoes in tropical and subtropical areas. Accurate diagnostic tests to differentiate the 2 infections are necessary for patient management and disease control. Using characterized ZIKV and DENV patient plasma in a blind manner, we validated an ELISA and a rapid immunochromatographic test for ZIKV detection. We engineered the ZIKV nonstructural protein 1 (NS1) for sensitive serologic detection with low cross reactivity against dengue and developed monoclonal antibodies specific for the ZIKV NS1 antigen. As expected, the serologic assays performed better with convalescent than acute plasma samples; the sensitivity ranged from 71% to 88%, depending on the performance of individual tests (IgM/IgG/NS1). Although serologic tests were generally less sensitive with acute samples, our ZIKV NS1 antibodies were able to complement the serologic tests to achieve greater sensitivity for detecting early infections. |
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| Keywords: | Zika dengue flavivirus viruses monoclonal antibody serologic test microcephaly immunochromatography diagnostics immunoglobulin enzyme-linked immunosorbent assay Zika virus |
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