针刺水沟穴抑制MCAO大鼠海马区神经细胞坏死的作用研究

目的:对比针刺水沟穴与水沟旁非穴对MCAO大鼠海马区坏死神经元作用。方法:将72只健康雄性Wistar大鼠按照随机数字表分为正常组、假手术组、模型对照组、非针刺组、水沟旁组、水沟组6组,每组12只。模型对照组、水沟旁组、水沟组参照Zea-Longa线栓法复制大鼠大脑中动脉缺血模型(MCAO)。水沟旁组、水沟组以频率180次/min、持续5s进行针刺干预。观察大鼠神经行为学,血流量,脑梗死面积比率,以及海马区神经细胞坏死率。结果:水沟组分别与非针刺组和水沟旁组比较差异都具有显著差异性P<0.05,而增加血流量方面水沟组也明显优于水沟旁组P<0.01。水沟旁组与非针刺组无显著性差异(P>0.05)。结论:针刺水沟穴抑制MACO大鼠神经细胞坏死,增加血流量从而降低梗死面积,而水沟旁无此作用.

Experimental Study on Shuigou(GV26)of Inhibiting Effect for Neuronal Necrosis of the Hippocampus

Objective:To investigate the effect of acupoint Shuigou(GV26)and the point side of Shuigou on inhibiting the neuronal necrosis of hippocampus induced by the middle cerebral artery obstruction(MCAO)in rats.Methods:Seventy-two adult Wistar rats were randomly divided into normal group,false-operation group,model group,none-acupuncture group,Shuigou-acupuncture group and side of Shuigou-acupuncture group,and 12 rats in each group.There were 4 groups established into the model of MCAO except normal group and false-operation group.Shuigou-acupuncture group and side of Shuigou-acupuncture group were needled with 2times/s for 5s respectively.Its effect was estimated by neurological behavior,cerebral blood flow,the rat of cerebral infarction area and the rat of neuronal necrosis of hippocampus.Results:Comparing with the side of Shuigou-acupuncture group and non-acupuncture group,Shuigou-acupuncture group was significantly increased on the neurological behavior and cerebral blood flow(P<0.05),decreased on the cerebral infarction area and the neuronal necrosis of hippocampus(P<0.05).There was no significant difference between non-acupuncture group and side of Shuigou-acupuncture group.Conclusion:Needling Shuigou(GV26)could inhibit the neuronal necrosis,but needling side of Shuigou could not.