抗柯萨奇B病毒性心肌炎胶囊复方提取物对病毒性心肌炎小鼠血清AST、LDH的影响

目的:观察抗柯萨奇B病毒性心肌炎胶囊复方(K-CoxB-JN)提取物对病毒性心肌炎小鼠心肌细胞的保护作用,并探讨其作用机制。方法:采用肢体Ⅱ导联心电图正常的雄性Balb/c小鼠腹腔注射CVB3,建立实验性小鼠病毒性心肌炎模型。将120只造模成功的雄鼠随机分模型组、黄酮低剂量组、多糖中黄酮中剂量组、原方水煎组,每组30只,各组小鼠在腹腔接种CVB3稀释液半小时后即给予相应药物(模型组灌胃生理盐水);另选取10只健康雄鼠作为正常对照组,腹腔接种同体积的生理盐水后给予生理盐水。将接种病毒当天定为第实验的1天,每日给药1次,连续给药14天,于第15天处死。观察小鼠生存状态及体重的变化,统计小鼠死亡率,计算心重指数,用分离的血清检测心肌酶CK、LDH的活性。结果:K-CoxB-JN各提取物组均能显著降低小鼠血清中AST、LDH的活性(P<0.05),改善小鼠心功能及病变小鼠体重情况、减少死亡率,其中多糖中黄酮中剂量组、原方水煎组效果最佳。结论:K-CoxB-JN提取物对病毒性心肌炎小鼠心肌细胞有很好的保护作用。

Effects of Compound Extracts from K-Coxb-JN on AST and LDH in Mice with Viral Myocarditis

Objective:To observe protection effect of compound extracts(K-CoxB-JN)from capsule for anti-viral myocarditis caused by coxsackie B3 virus(CVB3)on myocardial cells of mice with viral myocarditis,and to discuss possible mechanisms.Methods:Male Balb/c mice were injected intraperitoneally with coxsackie B3 virus.Model Mice with viral myocarditis were established successfully.120 model mice were divided into 4 groups randomly:blank model group,low-dose-flavone group,middle-dose-flavone group and original formula group,30 mice in each group.CVB3 was injected intraperitoneally in all mice except those in blank model group which were intragastricly administrated with normal saline.10 mice were selected as normal controlled group.Daily administration was once in each group for 14 days.All mice were killed in 15th day.Living condition and weights were observed,lethality rate was focused as well as index of cardial-weights.Finally,CK and LDH were determined with serum centrifuged.Results:Compound extracts can be used to drop down concentration of AST and LDH,there are significant differences(P<0.05).Compound extracts can improve cardial-function of mice and weights,lowing lethality rate,among which middle-dose and original formula have the better effects.Conclusion:K-CoxB-JN has fairly good protections on myocardial cells of model mice with viral myocarditis.