小鼠成红细胞成熟过程中乙酰胆碱酯酶合成情况的研究

将通过FVA病毒诱发小鼠髓外造血方法所获得的红系祖细胞进行培养,在不同时间收集原始、早幼、中幼及晚幼红细胞,分别进行乙酸胆碱酶(AchE)活性测定,并用~3H-肌醇标记细胞膜做SDS-聚丙烯酰胺凝胶电泳。结果表明原始红细胞有AchE活性,且电泳图谱中AchE处有~3H-放射活性;晚幼红细胞AchE活性及~3H-放射活性最高。提示AchE在红细胞形成的早期就已合成,并且已有肌醇磷脂与之结合而连接在膜上。

STUDY ON ACETYLCHOLINESTERASE SYNTHESIS DURING MATURATION OF MURINE ERYTHROBLASTS

Erythroblasts, obtained from the spleens of mice infected with anemia-inducing strain of Friend virus anemia (FVA), were cultured in Iscove's modified Dulbecco's medium (IMDM) with erythropoietin (Epo). Pro , basophilic, polychromatic and, orthochromatic normoblasts were collected from the cultures at different times. Activity of AchE was determined by chemical method. Cell membrane was incubated with 3H-inositol and analysed by SDS-PAGE. In pronormoblasts, AchE activity could already be detected and 3H-radioactivity appeared at the position corresponding to band 4.1 (where AchE was located). AchE activity and 3H- radioactivity reached the highest values at the stage of orthochromatic normoblasts. The results suggest that AchE may be synthesized at the very early stage of erythrocyte maturation and probably anchored on the plasma membrane through GPI.