PCNA与AgNORs在鼻咽癌组织中表达的定量研究

［目的］：为了增加鼻咽癌的诊断和预后判定指标。［方法］应用抗增殖细胞核抗原（抗ＰＣＮＡ）单克隆抗体ＰＣ１０，采用ＳＰ 免疫组化方法和 ＡｇＮＯＲｓ图像定量分析技术，对有确切随访结果的 ７８例鼻咽鳞状细胞癌和 ２２例鼻咽粘膜组织标本进行了定量 研究。［结果］经ＰＣＮＡ阳性反应计算出的增殖指数（ＰＩ）和图像分析的ＡｇＮＯＲｓ计数及其颗粒总面积均与ＮＰＣ分化程度高度相关， 随ＮＰＣ分化程度的增高而减少；ＰＣＮＡ阳性反应增殖指数与ＡｇＮＯＲｓ计数与５年生存率呈负相关（Ｐ＜０.０１）。［结论］认为ＰＣＮＡ的 表达和 ＡｇＮＯＲｓ图像分析技术有助于 ＮＰＣ分化程度及预后的判断。

Quantitative Study of PCNA Expression and AgNORs Image Analysis in Nasopharyngeal Carcinoma

Purpose] To search the factors of diagnosis and prognosis for nasopharyngeal carcinoma(NPC). [Methods] The expression of PCNA and AgNORs count or AgNORs area in 78 cases of nasopharyngeal squamous cell carcinoma and 22 cases of nasopharyngitis epithelia were quantitatively studied by SP immunostaining of monoclonal antitaly(PC-10) and image analysis system. [Results] The results showed that the proliferating index (PI) of PCNA positive cells and the AgNORs count or AgNORs area of NPC cells were both closely related to the degree of NPC histological differentiation,which decreased with the increasing degree of histological differentiation of NPC. Survival analysis showed that the PI of PCNA and the results of AgNORs count or area image analysis in surgical patients were also related to the 5 years survival rate. [Conclusions] The results suggest that immunostaining of PCNA and AgNORs image analysis are useful in evaluating the malignant degree and the prognosis of NPC.