CD4细胞计数,IL-6/IL-10比值联合对艾滋病合并肺孢子菌肺炎的诊断价值

目的探讨CD4细胞计数,IL-6/IL-10比值联合对艾滋病合并肺孢子菌肺炎的诊断价值。方法纳入2018年1月至2019年5月南充市中心医院收治的100例艾滋病合并肺孢子菌肺炎患者为艾滋肺炎组、100例艾滋病患者为艾滋组、100例健康体检者为对照组;通过流式细胞仪检测血清中CD4+T细胞的数量;酶联免疫吸附试验检测血清中IL-6,IL-10的表达水平。用受试者工作特征曲线(receiver operating characteristic curve,ROC)的AUC分析CD4细胞计数,IL-6/IL-10检测在艾滋病合并肺孢子菌肺炎中的诊断意义。结果艾滋病合并肺孢子菌肺炎患者血清中CD4细胞数量均<艾滋病患者与健康体检者(t=28.31,P<0.0001;t=36.90,P<0.0001),但是,IL-6/IL-10比值均高于艾滋病患者与健康体检者(t=7.184,P<0.0001;t=19.03,P<0.0001)。CD4细胞计数,IL-6/IL-10比值联合对艾滋病合并肺孢子菌肺炎的诊断的的灵敏度为92.00%,特异度为88.00%,准确度为89.33%。结论CD4细胞计数,IL-6/IL-10比值的检测可作为诊断艾滋病合并肺孢子菌肺炎的潜在标志。

Diagnostic value of CD4 cell count and IL-6/IL-10 ratio in combination for AIDS complicated with Pneumocystis pneumonia

Objective To explore the diagnostic value of CD4 cell count and IL-6/IL-10 ratio in combination for the diagnosis of AIDS complicated with Pneumocystis pneumonia.Methods A total of 100 AIDS patients with pneumocystis pneumonia admitted to the Nanchong Central Hospital from January 2018 to May 2019 were enrolled in the AIDS pneumonia group,100 AIDS patients were enrolled in the AIDS group,and 100 healthy subjects were included in the control group.The number of CD4+T cells in serum was detected by flow cytometry,and the expression levels of IL-6 and IL-10 in serum were detected by enzyme-linked immunosorbent assay.The AUC of receiver operating characteristic curve(ROC)was used to analyze the CD4 cell count and the diagnostic significance of IL-6/IL-10 detection in AIDS with pneumocystis pneumonia.Results The number of CD4 cells in the serum of AIDS patients with Pneumocystis pneumonia was significantly lower than that of AIDS patients and healthy subjects(t=28.31,P<0.0001;t=36.90,P<0.0001),but the ratio of IL-6/IL-10 was higher than that of AIDS patients and healthy individuals(t=7.184,P<0.0001;t=19.03,P<0.0001).The sensitivity of CD4 cell count and IL-6/IL-10 ratio in the diagnosis of AIDS patients with Pneumocystis pneumonia was 92.00%,the specificity was 88.00%,and the accuracy was 89.33%.Conclusion The detection of CD4 cell count and IL-6/IL-10 ratio can be used as a potential marker for the diagnosis of AIDS with Pneumocystis pneumonia.