Interleukin‐10‐secreting T cells define a suppressive subset within the HIV‐1‐specific T‐cell population |
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| Authors: | Eirik A. Torheim Lishomwa C. Ndhlovu Frank O. Pettersen Trine‐Lise Larsen Aashish R. Jha Knut M. Torgersen Dag Kvale Douglas F. Nixon Kjetil Taskén Einar M. Aandahl |
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| Affiliation: | 1. The Biotechnology Centre of Oslo and Centre for Molecular Medicine Norway, Nordic EMBL Partnership, University of Oslo, Oslo, Norway;2. Division of Experimental Medicine, Department of Medicine, University of California, San Francisco, CA, USA;3. Department of Infectious Diseases, Ullev?l University Hospital, University of Oslo, Oslo, Norway |
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| Abstract: | ![]()
Recent studies have indicated that Treg contribute to the HIV type 1 (HIV‐1)‐related immune pathogenesis. However, it is not clear whether T cells with suppressive properties reside within the HIV‐1‐specific T‐cell population. Here, PBMC from HIV‐1‐infected individuals were stimulated with a 15‐mer Gag peptide pool, and HIV‐1‐specific T cells were enriched by virtue of their secretion of IL‐10 or IFN‐γ using immunomagnetic cell‐sorting. Neither the IL‐10‐secreting cells nor the IFN‐γ‐secreting cells expressed the Treg marker FOXP3, yet the IL‐10‐secreting cells potently suppressed anti‐CD3/CD28‐induced CD4+ as well as CD8+ T‐cell proliferative responses. As shown by intracellular cytokine staining, IL‐10‐ and IFN‐γ‐producing T cells represent distinct subsets of the HIV‐1‐specific T cells. Our data collectively suggest that functionally defined HIV‐1‐specific T‐cell subsets harbor potent immunoregulatory properties that may contribute to HIV‐1‐associated T‐cell dysfunction. |
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| Keywords: | HIV antigens HIV type 1 IL‐10 Treg |
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