生黄合剂对缺血再灌注损伤大鼠心肌细胞凋亡相关基因Bax,Bcl-2和Caspase-3蛋白表达的影响

目的:观察生黄合剂对缺血再灌注损伤大鼠心肌细胞凋亡相关基因Bcl-2相关X蛋白(Bax)、B型白细胞/2型淋巴细胞样蛋白( Bcl-2)和天冬氨酸特异性半胱氨酸蛋白酶(Caspase-3)蛋白表达的影响.方法:将48只SD大鼠随机分为6组,即对照组、模型组、阳性药物组、生黄含剂低、中、高剂量组(17.5,35,70 mg·kg-1分别加入5 mL·kg-生脉饮),每组8只,分别给药7d后,建立心肌缺血再灌注损伤(MIRI)模型.采用原位末端标记法(TUNEL)检测心肌细胞凋亡情况,蛋白印迹法检测心肌细胞凋亡相关基因Bax,Bcl-2,Caspase-3蛋白的表达.结果:与模型组比较,生黄合剂能减少心肌细胞凋亡指数(AI)及Bax,Caspase-3蛋白的表达,增加Bcl-2蛋白的表达,提高Bcl-2/Bax的比值( P＜0.05),其中以生黄合剂高剂量组作用较为明显.结论:生黄合剂对大鼠心肌缺血再灌注损伤具有保护作用,其抗心肌细胞凋亡的机制可能与通过上调Bcl-2,下调Bax和Caspase-3基因表达,提高Bcl-2/Bax的比值有关.

Influence of Sheng-huang Mixtrue on Apoptosis Related Proteins Bax, Bcl-2 and Caspase-3 in Myocardial Ischemia-reperfusion Rats

Objective: To investigate the influence of Sheng-huang mixtrue on the apoptosis related proteins expression of Bcl-assosiated x protein(Bax), B-cell leukemia/lymphoma 2-like proteins(Bcl-2) and Aspartate-specific cysteinyl proteinase(Caspase-3) in myocardial ischemia-reperfusion injury rats. Method: SD rats(n=48) were divided randomly into six groups: normal control group, model group, positive control group, low-dose, medium-dose and high-dose Sheng-huang mixtrue groups(n=8). After the drug was administered to the six groups seven days, the Myocardial Ischemia-reperfusion injury in rats was established. TUNEL was used to detect the myocardial apoptosis index,The protein expressions of Bax, Bcl-2 and Caspase-3 were assayed by western blotting. Result: In comparison of the five other groups with model group,Sheng-huang mixture can decrease the myocardial apoptosis index and the protein expressions of Bax and Caspase-3, but increase the protein expressions of Bcl-2 in rats and the ratio of Bcl-2/Bax. Among which high dose Sheng-huang mixtrue group decreased much more than other groups. Conclusion: Sheng-huang mixture has the protective effects on myocardial ischemia-reperfusion injury in rats,which can inhibit the myocardial apoptosis of myocardial ischemia-reperfusion injury by increasing the protein expressions of Bcl-2 and reducing the protein expressions of Bax and Caspase-3.