趋化因子CXCL9在结直肠癌组织中的表达及意义

目的：通过检测趋化因子CXCL9在结直肠癌中的表达情况,探讨CXCL9与结直肠癌临床病理特征之间的关系。方法：72例结直肠癌患者按Dukes分期分为A、B、C、D 4组,应用Elisa法检测4组及对照组20例志愿者血清CXCL9的含量;应用免疫组织化学方法、Western Blot法检测72例结肠癌组织及癌旁组织中的CXCL9表达,探讨CXCL9与结直肠癌临床病理特征之间的关系。结果：4组结肠癌患者血清CXCL9的平均含量分别为10.21 ng/L1、7.12 ng/L、30.57 ng/L、56.26 ng/L,对照组血清CXCL9平均含量为7.83 ng/L,差异具有统计学意义（P〈0.05）。65.3%（47/72）的结肠癌组织CXCL9表达阳性;其中43.1%（31/72）为高表达,56.9%（41/72）为低表达,差异具有统计学意义（P=0.023）。11.1%（8/72）的癌旁组织CXCL9表达阳性,为低表达,与结肠癌比较,差异有统计学意义（P=0.000）。CXCL9的表达与结直肠癌患者的性别、年龄、肿瘤部位、肿瘤大小、组织类型无关（P〉0.05）;而与肿瘤的分化程度、淋巴转移、Dukes分期有关（P〈0.01）。结论：CXCL9在结直肠癌组织中呈较高表达,且表达水平与结直肠癌的浸润、转移等有关。

CXCL9 expression in colorectal cancer and its significance

Objective: To investigate the expression of chemokine CXCL9 in human colorectal cancer and explore its correlation to clinicopathologic features of colorectal cancer.Methods: 72 colorectal cancer patients were divided into four groups(A,B,C,D,respectively).Elisa was performed to detect the concentration of CXCL9 in 72 colorectal cancer patients and 20 volunteers' serum samples;Immunohistochemistry and Western-Blot were also performed to detect the expression of CXCL9 in 72 primary lesions of colorectal canc...