HBV宫内感染及其频率的研究

采用血清学(ELISA、RIA)、免疫组织化学(ABC法)和核酸分子杂交方法研究35例HBsAg阳性孕妇引产胎儿,并以10例HBsAg阴性孕妇引产胎儿为对照。结果从胎心血中检测到HBsAg和抗-HBcIgM,从胎肝组织査出HBcAg和HBVDNA证实胎儿在宫内感染了HBV。血清学、免疫组化和分子杂交检测,HBV宫内感染的检出率分别为20.0%(7/35)、5.9%(1/17)和44.4%(12/27),表明HBV宫内感染是HBV感染的重要途径。

Study on the HBV Intrauterine Infection and its Rate

In thirty-five fetuses induced from HBsAg-positive pregnant women, the fetuses were examined for HBV markers. Another 10 fetuses were used as control and none of the fetuses was infected by HBV in utero. Among 35 fetal serum samples, HBsAg and anti-HBcIgM were detected in 4 and 5 fetuses (in 7 fetuses) respectively. One out of 17 fetal liver rections was HBcAg positive by ABC stain. Twelve out of 27 fetal livers were HBV-DNA positive. Thus, the rate of intrauterine infection of HBV. was 20.0% (7/35), 5.9% (1/17) and 44.4% (12/27) respectively, by serological test, immunohistochemical test and molecular hybridization using P-labeled HBV DNA as a probe-The results showed that HBV intrauterine infection was an important mode of HBV transmission.