| 多对引物PCR方法鉴别尖锐湿疣和假性湿疣 |
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| 引用本文： | 刘跃华,王家璧,王晓峰,方凯. 多对引物PCR方法鉴别尖锐湿疣和假性湿疣[J]. 中国医学科学院学报, 1996, 0(4) |
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| 作者姓名： | 刘跃华 王家璧 王晓峰 方凯 |
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| 作者单位： | 中国医学科学院,中国协和医科大学,协和医院 |
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| 摘 要： | 应用多对引物ＰＣＲ方法对尖锐湿疣９５例和假性湿疣３３例进行了鉴别。结果９５例尖锐湿疣中８６例（９０．５％）ＨＰＶ６／１１ＤＮＡ阳性,３３例假性湿疣ＨＰＶＤＮＡ阴性,两组有非常显著差别（Ｐ＜０．００１）。本研究结果显示假性湿疣与ＨＰＶ无关。ＰＣＲ方法准确、省时、方便,是鉴别尖锐湿疣与假性湿疣的理想方法。
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| 关 键 词： | 尖锐湿疣 假性湿疣 人乳头瘤病毒 聚合酶链反应 |
Differentiation of Condylomata Acuminata from Pseudocondyloma by Using Multiple Primer Pairs Polymerase Chain Reaction |
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| Liu Yuehua,Wang Jiabi,Wang Xiaofeng, Fang Kai. Differentiation of Condylomata Acuminata from Pseudocondyloma by Using Multiple Primer Pairs Polymerase Chain Reaction[J]. Acta Academiae Medicinae Sinicae, 1996, 0(4) |
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| Authors: | Liu Yuehua Wang Jiabi Wang Xiaofeng Fang Kai |
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| Abstract: | Condylomata acuminata were differentiated from pseudocondyloma by using multiple primer pairs polymerase chain reaction(PCR)technique,The paraffin-embedded tissues of 95 cases of condylomata acuminata and 33 cases of pseudocondyloma were detected for HPV DNA. Results show that HPV 6/11 DNA are found in 86/95(90.5%)cases of condvlomata acuminata,and no HPV DNA is detected in 33 cases of pseudocondyloma,The results suggest that pseudocondyloma is not associated with HPV,Multiple primer pairs PCR technique is a preferable method for differentiating condylomata acuminata from pseudocondyloma. |
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| Keywords: | condylomata acuminata pseudocondyloma polymerase chain reaction human papilloma virus |
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