肝气郁结大鼠胃黏膜结构和功能变化的实验研究

目的:通过动物实验揭示肝郁及胃的病理生理基础,以指导临床辨证施治。方法:采用“束缚法”复制大鼠肝郁模型。14天后,观察正常组、肝郁组、复健组大鼠胃黏膜病理组织学变化,检测大鼠胃液中游离黏液量、前列腺素E2(PGE2)含量及胃黏膜组织匀浆液中超氧化物歧化酶(SOD)、一氧化氮(NO)水平。结果:肝郁组大鼠胃黏膜出现炎性改变,余两组无显著性变化;肝郁组大鼠胃液中游离黏液量、PGE2含量低于余两组;且胃黏膜组织匀浆液中的SOD、NO水平均低于余两组;复健组与正常组比较无显著差异。结论:肝郁可致胃黏膜产生炎性改变。胃液游离黏液量、PGE2含量及胃黏膜组织SOD、NO水平降低,是肝郁引起胃黏膜炎症的病理生理基础之一。疏肝解郁中药可有效预防肝郁及胃病理变化产生,内在机制与其能够调节上述指标,增强胃黏膜防御保护机能有关。

A Experimental Study on the Structural and Functional Changes of Gastric Mucosa in Rats with Liver- Qi Stagnation Syndrome

Objective:To reveal the pathophysiological foundations of Liver-Qi Stagnation affecting the stomach by animal experiment,and direct the clinical treatment.Methods: The model was reproduced by "bind method".60 healthy Wistar rats were divided into 3 groups randomly,male and female account for 50% respectively: control group(G1),model group(G2) and resumed group(G3).G3 were fed with herbal soup,G1 and G2 were fed with Saline in the same dose.14 days later,observed the pathologic changes of gastric mucosa,and determined the concentration of free mucus,PGE2 in gastric juice and the concentration of SOD,NO in gastric mucosa homogenate.Result:The pathomorphology of G2's gastric mucosa appeared inflammatory changes,G1 and G2 had no significant changes;The free mucus of G2 significantly lower than those of G1(P<0.01)and G3(P<0.05),G3's also lower than G1's,well without significant difference(P>0.05);The rank of the PGE2 level in every group's gastric juice was: G20.05);SOD and NO level in homogenate of gastric mucosa in G2 was significantly lower than those of G1(P<0.01)and G3(P<0.05),but those of G3 had no significant difference with G1(P>0.05).Conclusion: Liver-Qi Stagnation can lead to the inflammatory pathological changes in gastric mucosa.The reduction of concentration of free mucus,PGE2 in gastric juice and SOD,NO in gastric mucosa were one of the main pathophysiological foundations of the gastric mucosa inflammation caused by Liver-Qi Stagnation.The herbs of relieving depressed liver can prevent and treat stomach ailment that caused by Liver-Qi Stagnation,the inner mechanism was associated with its regulation to the aforesaid indices and its reinforce to the protective function of gastric mucosa.