滋补肝肾,益气活血方药对血管平滑肌细胞增殖及相关基因表 …

目的：采用血清药理学方法研究滋补肝肾、益气活血方药对内皮素（ＥＴ）诱导的自发性高血压大鼠（ＳＨＲ）和ＷＫＹ大鼠血管平滑肌细胞（ＶＳＭＣ）增殖及相关基因表达的影响。方法：采用＾３Ｈ－ＴｄＲ掺入实验检测ＶＳＭＣ增殖状态，Ｎｏｒｔｈｅｒｎ杂交，反转录－聚合酶链反应（ＲＴ－ＰＣＲ）检测中药血甭中ＶＳＭＣ诱导一氧化氮合酶基因和ＥＴ－诱导和ｃ－ｊｕｎ、增殖细胞怕（ＰＣＮＡ）基因表达活性的影响。结果：中导型一氧

Effect of Replenishing Liver and Kidney, Supplementing Qi and Activating Blood Circulation Recipe on Proliferation and Relevant Gene Expression of Vascular Smooth Muscle Cells

Objective: To study the effect of replenishing Liver and Kidney, supplementing Qi and activating blood circulation recipe (TCM) on the proliferation and relevant gene expression of vascular smooth muscle cells (VSMC). Methods: Using 3?H TdR incorporation to investigate the VSMC proliferation, using Northern blotting and reverse Polymerase Chain Reaction (RT PCR) to detect the effect of serum with TCM on induced NO synthase (iNOS), endothelin (ET) induced c jun and proliferation cell nucleus antigen (PCNA) gene expression. Results: The 3?H TdR incorporation value of VSMC treated by serum with TCM was lower than that of control serum (P<0 05). Rich iNOS mRNA was detected in the VSMC stimulated by serum with TCM. Expressions of c jun and PCNA gene induced by ET in the VSMC were significantly inhibited by serum with TCM. Conclusion: The TCM could significantly inhibit VSMC proliferation.