p16、Cyclin D1在增生性子宫内膜及子宫内膜癌中的表达

目的 :探讨 p16和 Cyclin D1在内膜癌发生、发展中的作用。方法 :采用免疫组化 S—P法对 12例正常子宫内膜、2 2例增生性子宫内膜及 4 1例子宫内膜癌中 p16和 Cyclin D1表达进行了研究。结果 :在单纯加复合增生、不典型增生及子宫内膜癌中 ,p16表达呈下降趋势 ,内膜癌与正常内膜及增生性内膜有显著性差异 (P<0 .0 1,P <0 .0 5 ) ;而Cyclin D1表达呈上升趋势 ,增生性子宫内膜、子宫内膜癌与正常内膜有显著性差异 (P<0 .0 5 ,P<0 .0 1)。不典型增生与单纯加复合增生 Cyclin D1过表达有显著性差异 (P<0 .0 1)。子宫内膜癌中 ,p16表达随细胞分化程度下降而降低 ,而Cyclin D1则随分化程度下降而上升 ,二者呈负相关。结论 :p16、Cyclin D1异常参与子宫内膜癌的发生 ;p16低表达、Cyclin D1过表达与内膜癌的恶性生物学行为有关 ;Cyclin D1核过表达可能是一个早期分子事件

The Expression of p16 and CyclinD1 in Proliferative Endometrium and Endometrial Carcinoma

Objectives:To study the role of p16 and cyclinD1 in the genesis and development of endometrial carcinoma.Methods:12 cases of normal endometrium,22 cases of proliferative endometrium and 41 cases of endometrial carcinoma were detected for the expression of p16 and cyclinD1 by means of immuniohistochemical S-P. Results:Among the groups of the simple and compound hyperplasia,the atypical hyperplasia and the endometrial carcinoma,p16 showed a descending tendency ,while the expression of cyclinD1 showed an ascending tendency.In endometrial carcinomas,the expression of p16 was significantly lower than normal endometrium and proliferative endometrium( P <0.01, P <0.05).However,the expression of cyclinD1 in proliferate endometrium and endometrial carcinoma was significantly higher than that in normal endometrium( P <0.05, P <0.01).The overexpression of cyclinD1 in the atypical hyperplasia group was obviously different from that in the simple and compound hyperplasia proup( P <0.01).In endometrial carcinoma,the expression of p16 was decreasing with the descending of cell differentiate degree,on the opposite,the expression of cyclinD1 was increasing,which indicated a negative correlation between them.Conclusions:The aberrant expression of p16 cyclinD1 was involved in the genesis of endometrial carcinoma;The low expression of p16 and the over expression of cyclinD1 correlate with the malicious biological behaviors of endometrial carcinoma;Over expression of cyclinD1 may be an earlier molecular event.