| 胃粘膜幽门螺杆菌多种方法检测及其比较分析 |
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| 引用本文： | 陆翠华 姚登福. 胃粘膜幽门螺杆菌多种方法检测及其比较分析[J]. 中国交通医学杂志, 1999, 13(4): 446-447 |
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| 作者姓名： | 陆翠华 姚登福 |
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| 作者单位： | 南通医学院附属医院 226001(陆翠华,姚登福,孟宪镛,葛政举,江枫),南通医学院附属医院 226001(施公胜) |
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| 摘 要： | 应用聚合酶链反应(PCR)扩增幽门螺杆菌(HP)特异性的DNA片段,并与组织学、涂片法、尿素酶法同步分析和比较.结果显示HP的PCR扩增产物经琼脂凝胶电泳,均为468bp的单一区带,与原设计一致;与组织学、涂片法和尿素酶法的同步检测,发现PCR法的敏感性为94.4%,明显高于组织学60.9%、涂片法77.8%和尿素酶法86.6%.提示PCR法能快速、敏感、特异地检测临床标本中的HP,对探讨HP与上消化道疾病之间的关系、指导抗菌治疗均具有重要意义.
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| 关 键 词： | 聚合酶链反应(PCR) 幽门螺杆菌 胃粘膜 |
MULTIASSAY DETECTION AND COMPARATIVE ANALYSIS OF HELICOBACTER PYLORI IN HUMAN STOMACH BIOPSIES |
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| Affiliation: | (Lu Cuihua.Yao Dengfu.Meng Xianyong.et al. Affiliated Hospital of Nantong Medical College,Nantong 226001) |
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| Abstract: | The specific DNA fragments of Helicobacter pylori were amplified in polymerase chain reaction assaywhich was compared with the assays of histology,smear and urease. The results revealed that a 468bp specific DNA product emerged by PCR amplification, consisting with the original design. The results showed that the sensitivity of the PCRassay was 94.4% , which was more than each of the assays of histology (60.9%) ,smear(77.8% ) and urease(86. 6%).The data suggested that the PCR assay could detect HP in clinical samples rapidly, sensitively and specifically. It is veryuseful for discussing the relationship between HP infection and the upper digestive tube diseases and guiding the treatmentof and - HP. |
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| Keywords: | Polymerase chain reaction Helicobacter pylori Stomach biopsies |
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