正交投影鉴别决明子色谱指纹图谱峰纯度

应用正交投影法对色谱指纹图谱的峰进行鉴别。用对照组分光谱与目标色谱峰前后的零浓度区光谱构建正交投影阵,对目标色谱峰的光谱进行投影,以投影前后光谱的夹角余弦作为判据来鉴别目标色谱峰的峰纯度。对决明子药材色谱指纹图谱的峰纯度鉴别,识别出大黄素、大黄素甲醚及大黄酚三个纯色谱峰。正交投影鉴别色谱指纹图谱峰的纯度方法结果可靠,背景的干扰可消除。

Identification Peak Purity of Chromatographic Fingerprints Based on Orthogonal Projection

A procedure for identification peak purity of chromatographic fingerprints by orthogonal projection is developed.The procedure consists of several steps: first,the projection matrix was composed with the spectrum of the standard component and the spectrum of zero-component regions before and after the target component peak,and then the target component spectrum is multiplied by the projection matrix,finally,cosine value of between target component spectrum and its projected residue is applied as a criterion to test the peak purity of target components.Three pure peak,emodin,physcion and chrysophaol of chromatographic fingerprints of Cassiae Semen crude drugs,was identified by the proposed method.Experiment results show the method of identification peak purity of chromatographic fingerprints by orthogonal projection is reliable;the effect of spectral and chromatographic background can be corrected.