膀胱移行上皮癌细胞凋亡和增殖细胞核抗原表达研究

目的研究表浅膀胱移行细胞癌中细胞凋亡及增殖细胞核抗原（ＰＣＮＡ）表达情况,并初步探讨其与表浅膀胱移行细胞癌复发的关系。方法采用原位ＤＮＡ末端标记法及免疫组织化学方法检测了８７例表浅膀胱移行细胞癌中细胞凋亡及ＰＣＮＡ表达情况,计算出细胞凋亡指数及ＰＣＮＡ表达指数,并对复发组与非复发组进行比较。结果复发组与非复发组细胞凋亡指数差异无显著意义,而凋亡指数与ＰＣＮＡ指数比值在复发组小于非复发组（Ｐ＜００１）,显示在复发组癌细胞的增殖程度明显高于细胞凋亡程度。结论对凋亡指数及ＰＣＮＡ阳性指数的综合分析,即凋亡指数与ＰＣＮＡ阳性指数的比值,可更全面的反映细胞凋亡与增殖的平衡状态,对判断肿瘤的复发具有参考价值。

Apoptosis and expression of PCNA in superficial transitional cell bladder cancer as related to recurrence

OBJECTIVE: To determine the expression of apoptosis and PCNA in superficial transitional cell bladder cancer and to evaluate its significance as a recurrence marker. METHOD: 87 cases of superficial transitional cell bladder cancer were analysed for the expression of apoptosis and PCNA by using the 3-end labeling method of DNA and immunohistochemical staining in tissue sections. The apoptotic indexes and PCNA indexes were respectively the percentages of apoptotic cells or PCNA positive cells among tumor cells. RESULT: The end labeling method allowed a precise evaluation of the expression of apoptosis. Apoptosis occurred in 89.6% of superficial transitional cell bladder cancer. In 17 patients who suffered from tumor recurrence, the ratio of apoptosis indexes to PCNA indexes was significantly higher than that in those without recurrence (P < 0.01). CONCLUSION: The ratio of apoptosis indexes to PCNA indexes was believed to be related to tumor recurrence and might be of value in the assessment of recurrence of superficial transitional cell bladder cancer.