X射线照射诱导膀胱癌细胞凋亡的研究

目的 探讨Ｘ射线照射对膀胱癌细胞系存活状态的影响。方法 应用荧光染料（ＥＢ和ＡＯ）染色、荧光显微镜观察法，对膀胱癌ＥＪ和ＢＩＵ－８７细胞用不同剂量Ｘ射线照射及照射后不同时间收集细胞检测。结果 Ｘ射线照射可诱导诱胱癌细胞凋亡和坏死，细胞凋亡和坏死的多少与照射剂量及照射后的时间长短有关。在照射１００－１０００ｃＧＹ时凋亡细胞数明显增多，照射１０００￣２０００ｃＧＹ时坏死细胞数明显增多，随照射剂量增加和

Apoptosis induced by ionizing radiation in TCC cell lines,EJ and BIU 87

Objective To study the effect of ionizing radiation on the living status of TCC cell lines, EJ and BIU 87. Methods Different doses of X ray were used on cell lines,EJ and BIU 87, and the cells were observed microscopically by fluorescent staining(EB and AO).Results The number of normal living cells declined and apoptotic as well as necrotic cells elevated with increase of radiation doses except for doses equal to or below 100 cGY, Apoptotic and necrotic cells increased as postradiation time delayed.Conclusion The ionizing radiation may induce TCC apoptosis and necrosis.