燃煤型氟中毒对雌鼠卵巢生殖功能影响

目的 动态观察燃煤型氟中毒对雌鼠卵巢生殖功能影响。方法 SD雌鼠自由食用织金病区原煤烘烤的玉米饲料复制燃煤型氟中毒动物模型;动态观察雌鼠染氟期间牙齿的变化情况;测定大鼠尿氟、骨氟含量;分离卵巢计算卵巢系数,光镜下观察卵巢组织形态、各级卵泡及黄体的数量变化;于不同时间段(60、120、180 d)动情期处死雌鼠,分离血清,放射免疫法检测血清中雌二醇含量。结果 与对照组比较,随染氟时间增加,染氟组雌鼠尿氟与骨氟含量明显升高,呈时间效应关系;各染氟组雌鼠卵巢卵泡颗粒细胞呈现渐进性水肿变化,闭锁卵泡和黄体退化逐渐增多,成熟卵泡偶见;与对照组比较,中氟组雌鼠染氟180 d闭锁卵泡数量[(24.700±4.138)个]与高氟组雌鼠染氟120、180 d时闭锁卵泡数量[分别为(24.200±3.706)、(25.000±4.714)个]明显升高(P<0.05);与对照组比较,染氟60 d时,中、高氟组雌鼠血清中雌二醇含量[分别为(43.875±7.180)、(45.625±5.780)pmol/L]升高(P<0.05)。结论 燃煤型氟中毒对雌鼠卵巢具有毒性作用,其机制可能与加速各级卵泡退化、闭锁、导致卵泡颗粒细胞水肿、卵泡细胞受损,进而影响雌二醇合成和分泌有关。

Effects of fluorosis caused by coal-burning on ovarian reproductive function in female rats

Objective To observe influences of fluorosis caused by coal burning on ovarian reproductive function in female rats.Methods Female Sprague-Dawley rats in experiment group were fed with corn roasted with raw coal from Zhijin area,an endemic fluorosis area,to establish an animal model of coal-burning fluorosis.The changes of the teeth of the female rats during fluorine exposure was observed.Fluorine contents of urine and bone were detected.The ovaries of the rats were collected and ovary coefficient was calculated;the pathological morphology of ovary,the changes of ovarian follicles at different development stage,and the number of corpus luteum were observed with light microscope.Estrus female rats were sacrificed at different time(60,120,180 days)of the fluoride exposure to collect serum samples for the determination of estradiol(E₂)with radioimmunoassay.Results Compared with the control group,the fluorine contents of urine and bone increased significantly with the increment of fluoride exposure time in the experiment group.In the fluoride exposure group,all follicular granulosa cells showed progressive edema;atresia of the follicle and degradation of corpus luteum increased gradually and mature follicle were observed occasionally.Compared with the control group,the number of atresia follicles significantly increased in moderate fluoride exposure group(24.000±4.138 at 180 day)and in high fluride exposure group(24.200±3.705 at 120 day and 25.000±4.714 at 180 day)(P<0.05 for all).Compared with the control group,serum estradiol levels in the rats in moderate fluoride exposure group(43.875±7.180 pmol/L)and high fluoride exposure group(45.625±5.780 pmol/L) increased at 60 day of the experiment(P<0.05 for all).Conclusion There are toxic effects of coal-burning fluorosis on the ovary of female rasts and the mechanism of the effects is related to the increases of follicle degeneration and atresia of follicles at different stages,follicular granulosa cell edema,damage of follicular cells,and the impaired synthesis and secretion of E₂.