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| 5-脂氧合酶激活蛋白抑制剂MK886对人食管癌细胞系增殖和凋亡的影响 | |
| 引用本文： | 史铁伟,李天柱,周静,娜日苏,孙琪,许丽艳,白春英. 5-脂氧合酶激活蛋白抑制剂MK886对人食管癌细胞系增殖和凋亡的影响[J]. 基础医学与临床, 2022, 42(1): 56-61. DOI: 10.3969/j.issn.1001-6325.2022.01.010 |
| 作者姓名： | 史铁伟 李天柱 周静 娜日苏 孙琪 许丽艳 白春英 |
| 作者单位： | 赤峰学院 基础医学院 内蒙古人类遗传病研究重点实验室,内蒙古 赤峰024000,汕头大学 医学院 潮汕沿海地区高发肿瘤分子生物学广东省高校重点实验室,广东 汕头515000 |
| 基金项目： | 国家自然科学基金(81860496);赤峰学院青年基金(cfxyqn201908);赤峰学院大学生创新创业训练计划(2019-09自治区级);内蒙古自治区高等学校科学技术研究项目(NJZY20203)。 |
| 摘 要： | 目的 5-脂氧合酶活化蛋白（FLAP）抑制剂MK886对人食管癌细胞系（KYSE-150和TE-3）增殖和凋亡的影响及作用机制。方法用2.5、5、10、20、40和80μmol/L的MK886干预体外培养的KYSE-150和TE-3细胞;xCELLigence RTCA系统实时测定细胞增殖抑制率,同时确定半数抑制浓度(IC50)。流式细胞测量术检测食管癌细胞的细胞周期。Western blot检测细胞凋亡和自噬相关蛋白的表达。结果人食管癌细胞系（KYSE-150和TE-3）的增殖抑制率随着MK886浓度增加而增强（P<0.05）,KYSE-150组的IC50浓度为29.11μmol/L,TE-3组的IC50浓度为27.47μmol/L。当MK886浓度增加至25μmol/L时,食管癌细胞G0/G1期滞后增加明显（P<0.001）,MK886处理浓度增加到50μmol/L时,食管癌细胞G2/M期增加明显（P<0.001和P<0.05）... |
| 关 键 词： | 食管癌 增殖 凋亡 MK886 脂氧合酶 |
Effects of 5-lipoxygenase activating protein inhibitor MK886 on proliferation and apoptosis of human esophageal cancer cell lines |
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| SHI Tie-wei,LI Tian-zhu,ZHOU Jing,NA Ri-su,SUN Qi,XU Li-yan,BAI Chun-ying. Effects of 5-lipoxygenase activating protein inhibitor MK886 on proliferation and apoptosis of human esophageal cancer cell lines[J]. Basic Medical Sciences and Clinics, 2022, 42(1): 56-61. DOI: 10.3969/j.issn.1001-6325.2022.01.010 | |
| Authors: | SHI Tie-wei LI Tian-zhu ZHOU Jing NA Ri-su SUN Qi XU Li-yan BAI Chun-ying |
| Affiliation: | (Key Laboratory of Human Genetic Diseases in Inner Mongolia, School of Basic Medicine,Chifeng University, Chifeng 024000;Key Laboratory of Molecular Biology of High Incidence Tumor in Chaoshan Coastal Area of Guangdong Province,Medical College,Shantou University,Shantou 515000,China) |
| Abstract: | Objective To investigate the effects of 5-lipoxygenase activating protein(FLAP)inhibitor MK886 on the proliferation and apoptosis of human esophageal carcinoma cell lines(KYSE-150 and TE-3)and to explore the potential mechanism.Methods KYSE-150 and TE-3 cells were treated with MK886(2.5,5,10,20,40 and 80μmol/L).xCELLigence RTCA system was used to to detect the half maximal inhibitory concentration(IC50)of MK886.The expressions of proteins with apoptosis and autophagy were detected by Western blot and cell cycles were detected by flow cytometry.Results MK886 decreased the proliferation of human esophageal carcinoma cells(KYSE-150 and TE-3)(both P<0.05).IC50 of MK886 in KYSE-150 group was 29.11μmol/L and in TE-3 group of 27.47μmol/L.The proportion of G0/G1 phase was increased(both P<0.001)However,in the group treated with concentrations of MK88650μmol/L,G2/M phase was increased(P<0.05).The expression of cleaved-caspase-3 and LC-3A/B-Ⅱwere increased with MK886(both P<0.001).Conclusions MK886 inhibits proliferation of human esophageal carcinoma cell lines and induces apoptosis. |
| Keywords: | esophageal carcinoma proliferation apoptosis MK886 lipoxygenase |
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