| 细菌脂多糖诱导小鼠DC2.4细胞TLR7蛋白的表达 |
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| 引用本文： | 杨恒丽,姬新颖,王平忠,潘蕾,连建奇,郝春秋,王伟,贾战生.细菌脂多糖诱导小鼠DC2.4细胞TLR7蛋白的表达[J].细胞与分子免疫学杂志,2008,24(5):438-440. |
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| 作者姓名： | 杨恒丽 姬新颖 王平忠 潘蕾 连建奇 郝春秋 王伟 贾战生 |
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| 作者单位： | 第四军医大学唐都医院全军感染病诊疗中心,陕西,西安,710038 |
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| 摘 要： | 目的:探讨细菌脂多糖(LPS)对小鼠树突状细胞(DC)系DC2.4 TLR7蛋白表达的诱导作用.方法:用RPMIl640完全培养液培养DC2.4细胞,光学显微镜观察LPS刺激前后的细胞形态特征,RT-PCR和Western blot分别测定TLR7 mRNA和蛋白表达.结果:LPS刺激前后,细胞中均有TLR7 mRNA转录.LPS刺激前,细胞较小而透亮,树突较少,无,TLR7蛋白表达;LPS刺激后,细胞体积增大,树突增粗增多,刺激后12 h、24 h、48 h,TLR7蛋白均有表达,且在3个时间点的表达量基本一致.结论:LPS可诱导DC2.4中TLR7蛋白的表达.
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| 关 键 词： | 脂多糖 DC2.4细胞 TLR7 细菌脂多糖 脂多糖诱导 小鼠树突状细胞 蛋白表达 表达量 lipopolysaccharide bacterial induced protein expression 时间点 增粗 体积增大 转录 结果 mRNA 测定 Western blot 形态特征 刺激 |
| 文章编号： | 1007-8738(2008)05-0438-03 |
| 修稿时间： | 2007年10月22 |
TLR7 protein expression induced by bacterial lipopolysaccharide in DC2.4 cells |
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| YANG Heng-li,JI Xin-ying,WANG Ping-zhong,PAN Lei,LIAN Jian-qi,HAO Chun-qiu,WANG Wei,JIA Zhan-sheng.TLR7 protein expression induced by bacterial lipopolysaccharide in DC2.4 cells[J].Journal of Cellular and Molecular Immunology,2008,24(5):438-440. |
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| Authors: | YANG Heng-li JI Xin-ying WANG Ping-zhong PAN Lei LIAN Jian-qi HAO Chun-qiu WANG Wei JIA Zhan-sheng |
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| Institution: | Center of Diagnosis and Treatment for Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. |
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| Abstract: | AIM: To investigate the role of bacterial Lipopolysaccharide (LPS) in inducing TLR7 protein expression in DC2.4 cells. METHODS: DC2.4 cells were cultured in RPMI1640 medium supplemented with 100 mL/L FBS. Optical microscope was used to observe the cells morphology before and after LPS induction. RT-PCR and Western blot were employed to analyze the expression of TLR7 at mRNA and protein lever, respectively. RESULTS: Without LPS induction, DC2.4 cells were small and shine with a few dendrites, didn't express TLR7 protein. However, DC2.4 cells become bigger and the dendrites of them become more robust with LPS induction. DC2.4 cells start to express TLR7 protein after 12 hours LPS induction, and there is no much difference on TLR7 protein expression after 12 h, 24 h, 48 h LPS stimulation. CONCLUSION: LPS can induce TLR7 protein expression in DC2.4 cells. |
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