口腔鳞状细胞癌临床特征与端粒酶相关性研究

目的 :了解口腔鳞状细胞癌组织中端粒酶的活性及其与主要临床特性之间的相关性。　方法 :采用PCR ELISA法对不同TNM分期的 4 5例口腔鳞状细胞癌组织标本、12例口腔正常粘膜组织标本行端粒酶活性检测。　结果 :口腔鳞状细胞癌组端粒酶活性阳性率 (75 .6 7% )明显高于口腔正常粘膜组 (0 .0 0 % ) (P <0 .0 1)。口腔鳞状细胞癌临床晚期 (TNMⅢ、Ⅳ期 )端粒酶活性阳性率 (95 .0 0 % )明显高于临床早期 (TNMⅠ、Ⅱ期 ) (P <0 .0 5 ) ,伴颈淋巴结转移组端粒酶活性阳性率 (10 0 % )明显高于不伴颈淋巴结转移组 (6 3.33% ) (P <0 .0 5 )。　结论 :端粒酶活性与口腔鳞状细胞癌的临床分期及病变进程密切相关。

To study on the relation between clinical character and telomerase in oral squamous cell carcinoma

Objectives:To find out the relation between main clinical character and telomerase activity in oral squamous cell carcinomas. Methods:Telomerase activity was detected by telomerase PCR ELISA assay in 12 cases of oral normal tissue and 45 cases of oral squamous cell carinoma. Results:Telomerase activity was not detectable in 12 cases of oral normal tissue. Telomerase activity was detected in 75.67 percent of oral squamous cell carcinomas. Telomerase activity was present more frequently in advanced oral squamous cell carinoma than in the early stage( P <0.05),and more frequently in patients with positive lymph node metastasis than in those without lymph node metastasis( P <0.05). Conclusions:Telomerase activity has important effect in the progress of oral oncogenesis. There are close relation between clinical character and telomerase activity in oral squamous cell carinoma.