通心络胶囊对家兔髂股动脉粥样硬化闭塞症的影响

目的：观察通心络胶囊对家兔髂股动脉粥样硬化闭塞症(ASO)的影响。方法：将家兔随机分为假手术组,模型组,通心络胶囊0.8,0.4,0.2 g·kg^-1剂量组,通塞脉片0.8 g·kg^-1剂量组及来适可0.002 g·kg^-1剂量组；采用机械损伤、免疫损伤及高脂饲料喂养等方法复制家兔髂股动脉粥样硬化闭塞症模型；造模8周开始给药,实验前(0周)、给药前(术后8周)、给药4周(术后12周)、给药8周(术后16周)、给药12周(术后20周)测定血液中血脂(TC,TG,HDL-C,LDL-C)水平,进行行为学观察, 并测定术侧后肢远端皮肤温度；实验结束时造影,并取术侧髂股动脉进行组织病理学检查。结果：通心络胶囊明显降低动脉粥样硬化闭塞症家兔血清TC,TG,LDL-C,VLDL-C水平及TC/HDL-C；明显改善术侧后肢跛行程度,增加术侧后肢远端皮温；减少术侧后肢髂股动脉内膜厚度和内膜面积比率,减轻术侧后肢髂股动脉管腔狭窄程度。结论：通心络胶囊具有调节血脂、防治ASO的作用。

Effects of Tongxinluo capsule on atherosclerosis obliterans in iliofemoral artery of rabbits

Objective: To explore the effects of Tongxinluo capsule (TXL) on the atherosclerosis obliterans (ASO) in iliofemoral artery of rabbits. Method: Rabbits were randomly divided into 7 groups: sham, model, TXL (0.8, 0.4, 0.2 g·kg^-1), Tongsaimai tablet (0.8 g·kg^-1) and Laishike (0.002 g·kg^-1). The animal model of ASO was established with a combined method of mechanical trauma, immunologic injury and high fat fodder feeding. Rabbits were administrated the drugs 8 weeks after surgery. The levels of TC, TG, HDL-C and LDL-C in serum were determined at the time points below: pre-experiment (0 week), pre-drug administration (8 weeks post-surgery), 4 weeks after drug administration (12 weeks post-surgery), 8 weeks after drug administration (16 weeks post-surgery), 12 weeks after drug administration (20 weeks post-surgery). Meanwhile, the behavioral study was performed, the distal skin temperature of the injured hind limb detected. The histopathological changes in iliofemoral artery were examined after opacification. Result: The levels of TC, TG, LDL-C, VLDL-C and TC/HDL-C were decreased significantly in serum of ASO rabbits. The severity of lameness in the injured hind limb was improved. The distal skin temperature was increased. The thickness and the ratio of intima area of the iliofemoral artery of the injured hind limb were decreased, while the stenosis extent was improved. Conclusion: TXL might be beneficial to modulate blood lipid, as well as the prevention and treatment for ASO.