美洲大蠊多肽PAE2逆转BEL-7402/5-FU细胞多药耐药性的作用及机制研究

本研究探讨了美洲大蠊多肽PAE2逆转人肝细胞肿瘤耐药细胞株BEL-7402/5-FU的多药耐药性作用及机制.以人肝细胞肿瘤敏感细胞株BEL-7402、人肝细胞肿瘤耐5-氟尿嘧啶(5-FU)细胞株BEL-7402/5-FU和Balb/c-nude小鼠为研究对象,采用噻唑蓝比色分析法检测BEL-7402/5-FU 细胞的多...

Effect and Mechanism of the Periplaneta American Polypeptide PAE2 on Reversing Multi-Drug Resistance of BEL-7402/5-FU Cells

The effect and mechanism of periplaneta American polypeptide PAE2 in reversing the multi-drug resistance of hepatocellualar carcinoma resistant BEL-7402/5-FU cells were studied in this paper.Hepatocellualar carcinoma sensitive cells of BEL-7402,hepatocellualar carcinoma resistant 5-fluorouracil(5-FU)cells of BEL-7402/5-FU and Balb/c-nude mice were used as the research objects.MTT method was used to detect the multi-drug and crossdrug resistance of BEL-7402/5-FU cells,and the accumulation of doxorubicin in BEL-7402/5-FU cells was observed by laser confocal microscope.In order to determine the mechanism of the periplaneta American polypeptide PAE2 in reversing multi-drug resistance,real-time fluorescent quantitative polymerase chain reaction and immunocytochemical staining method were used to detect the mRNA and protein relative expression of P-glycoprotein 1(P-gp1),multi-drug resistance protein 1(MRP1),lung resistance protein 1(LRP1)and breast cancer resistance protein 1(BCRP1)in BEL-7402/5-FU cells.In addition,an axillary tumor model of BEL-7402/5-FU cells was established in Balb/c-nude mice to observe the effects of periplaneta American polypeptide PAE2 on immune organs,liver and kidney function,tumor inhibition and induction of tumor cell apoptosis,as well as regulation of multi-drug resistance related proteins in tumor tissues.The results showed that the periplaneta American polypeptide PAE2 could effectively reverse the multi-drug and cross-drug resistance of BEL-7402/5-FU cells.The mechanism might be related to the down-regulation of the expression of multi-drug resistance related proteins,which reduced the efflux ability of multi-drug resistant cells on anti-tumor drugs and intervene molecular metabolism of anti-tumor drugs,reversing the multi-drug resistance of hepatocellualar carcinoma finally.