乙肝HBV DNA荧光定量检测与血清标志物结果相关性分析

目的 研究乙肝血清学标志物（HBV-M）结果与HBV-DNA水平的关系，分析HBV DNA阳性率在各年龄组的分布情况。方法对深圳市部分服务行业人员血清应用荧光定量PCR检测和ELISA法检测并对结果进行分析。结果1781例乙肝血清学标志物阳性血清中有1102例HBV-DNA阳性。其中883例1．3．5阳性（俗称“大三阳”）血清中HBV-DNA的阳性率为96．15％；722例1．4．5阳性（俗称“小三阳”）血清中HBV-DNA的阳性率为24．38％；138例1．5阳性血清中HBV-DNA的阳性率为38．41％；22例1．3阳性血清中HBV-DNA的阳性率为90．91％；11例HBsAg阳性血清中HBV-DNA阳性率为36．36％。结论两种方法检测乙肝标志物有密切的相关性。荧光定量PCR是检测HBV-DNA含量较精确的方法，能正确反映乙肝病毒的复制水平和活跃程度；HBsAg和HBeAg与HBV-DNA含量有着明显的相关关系。

Analysis of correlation of quantitative fluorescent detection of HBV DNA and the results of serum marker.

Objective To analyze the relationship between the level of HBV DNA and the HBV-M. Methods Serum samples from 1 781 service workers were detected by ELISA and divided into nine groups: HBsAg(+)/HBeAg(+)/HBcAb(+),HBsAg(+)/HBeAb(+)/HBcAb(+),HBsAg(+)/HBcAb(+),HBsAg(+)/HBeAg(+),HBsAb/HBcAb(+),HBeAb(+)/HBcAb(+),HBsAg(+)/HBeAb(+),HBsAb(+) and HBsAg(+).HBV DNA concentration was determined by fluorescent quantitative PCR. Results The positive rate of HBV-DNA in the group of HBsAg(+)/HBeAg(+)/HBcAb(+) was 61.88%(1 102/1 781),the positive rate in the group of HBsAg(+)/HBeAb(+)/HBcAb(+) was 96.15%,the positive rate in the group of HBsAg(+)/HBeAb(+)/HBcAb(+) was 24.38%,the positive rate in the group of HBsAg(+)/HBcAb(+) was 38.41%,90.91% in the group of HBsAg(+)/HBeAg(+),36.36% in the group of HBeAg(+) and the positive rate of HBV-DNA was 0% in the other groups.There significant differences between the different hepatitis B sera positive modes and the result of HBV DNA.The HBV DNA level showed an obvious correlation with the HBeAg content in sera. Conclusion A close correlation exists between these two methods in the detection of HBV markers.The FQ-PCR is a relatively acurate method for detection of HBV-DNA.