槐耳清膏对结肠癌SW480细胞增殖能力影响及其机制探讨

目的：探索槐耳清膏对体结肠癌SW480细胞增殖能力影响及其机制。方法：采用噻唑蓝（MTT）比色法检测槐耳清膏对SW480细胞增殖能力的作用,并探求最佳作用浓度;将体外培养细胞随机分为常氧组（NC组）、低氧组（HC组）和低氧槐耳组（HH组）,逆转录聚合酶链反应（RT PCR）检测各组血管内皮生长因子（VEGF）mRNA表达水平,Western blot检测蛋白表达水平。结果：槐耳清膏对SW480细胞抑制率随药物浓度增加而上升,1 mg/mL时抑制率最大（66.7%）,与氟尿嘧啶组（浓度为10μg/mL）相比无统计学意义。HH组和HC组VEGF mRNA表达均显著高于NC组,分别为4.71±0.07,4.54±0.02和1.19±0.03（P〈0.05）,但HH组与HC组比较差异无统计学意义。HC组VEGF蛋白表达显著高于NC组,分别为0.66±0.03和0.38±0.02（P〈0.05）,HH组较HC组VEGF蛋白表达均显著下降,分别为0.37±0.03和0.66±0.03（P〈0.05）。结论：槐耳清膏可抑制SW480细胞增殖,1 mg/mL时抑制率最大。其机制为槐耳清膏下调细胞内VEGF蛋白表达,从而抑制肿瘤生长。

Effect of Huaier Cream on Proliferation of Colon Cancer Cells SW480 and its Mechanism

Objective：To investigate the effect of Huaier cream on proliferation of colon cancer cells SW480 and its mechanism.Methods：The proliferation was analyzed by MTT.SW480 cells were randomly divided into normoxic group（NC group）,hypoxia group（HC group） and hypoxia group treated by Huaier（HH group）.Levels of mRNA and protein expression of VEGF were detected by RT-PCR and Western blot,respectively.Results：Huaier cream induced a dose-dependent inhibition of SW480 cells.The maximum percentage of growth inhibition was 66.7%at a concentration of 1.0 mg/mL,but no significant difference was found compared to the positive control（5-FU 10μg/mL）. VEGF mRNA levels were significantly higher in HC group and HH group than in NC group（4.71±0.07,4.54±0. 02 vs 1.19±0.03,all P0.05）,but not significantly different between HC group and HH group.VEGF protein expression was higher in HC group than NC group（0.66±0.03 vs 0.38±0.02,P0.05）.In HH group,VEGF protein was inhibited remarkably compared with HC group（0.37±0.03 vs 0.66±0.03,P0.05）.Conclusion：Huaier cream can significantly inhibit SW480 cells and the top inhibition concentration is 1.0 mg/mL.Huaier cream plays a role in inhibiting tumor through down-regulating protein expression of VEGF.