| MDL28170对缺氧缺血新生鼠大脑神经细胞凋亡的影响 |
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| 引用本文： | 陈莉娜,陈大鹏,姚裕家.MDL28170对缺氧缺血新生鼠大脑神经细胞凋亡的影响[J].华西医学,2009(12). |
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| 作者姓名： | 陈莉娜 陈大鹏 姚裕家 |
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| 作者单位： | 四川大学华西第二医院儿科 |
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| 摘 要： | 目的:探讨卡配因抑制剂-3(MDL28170)对新生大鼠缺氧缺血性脑损伤(HIBD)神经细胞凋亡的影响。方法:建立新生SD大鼠HIBD模型,治疗组于缺养缺血后即刻、2 h、4 h腹腔内注射MDL28170,对照组及手术组同时予生理盐水。缺氧缺血后24 h用免疫组化方法观察大脑皮质及海马CA1区Caspase-3蛋白表达、TUNEL法检测细胞凋亡,观察组织病理改变并计算海马神经元死亡数,透射电镜观察细胞超微结构。结果:缺氧缺血后24 h缺血侧大脑皮质及海马CA1区Caspase-3和TUNEL阳性细胞数较对照组明显增加,透射电镜证实有凋亡细胞;MDL28170可减少阳性细胞数量,抑制神经元死亡,差异有显著性(P<0.05)。结论:MDL28170可通过抑制神经凋亡而对新生大鼠HIBD具有一定保护作用。
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| 关 键 词： | 大鼠 新生 缺氧缺血性脑损伤 凋亡 卡配因抑制剂-3(MDL28170) |
Effect of MDL28170 on Neural Apoptosis after Hypoxic-Ischemic Brain Damage in Neonatal Rats |
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| Institution: | CHEN Li-na; CHEN Da-peng; YAO Yü-jia.the Department of Pediatrics; West China Second University Hospital; Sichuan University; Sichuan 610041; China; |
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| Abstract: | Objective: To investigate the effect of(Calpain inhibitor-3) MDL28170 on neural apoptosis in a neonatal model of hypoxic-ischemic brain damage(HIBD).Methods: A neonatal model of HIBD was established,7-day-old SD rats were divided into three groups.The treatment group received MDL28170(ip) at 0 h,2 h,4 h after HI,whereas the other two groups were administered normal saline simultaneously.The expression of caspase-3(by immunohistochemistry),neural apoptosis(by TUNEL) in cortex and hippocampus ipsilateral to t... |
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| Keywords: | rat neonate hypoxic-ischemic brain damage apoptosis calpain inhibitor-3（MDL28170） |
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