脑胶质瘤SOX7基因的甲基化状态及临床意义

目的 探讨脑胶质瘤SOX7基因的甲基化状态及其与病人预后的关系。方法 收集2013年6月至2015年1月手术切除的胶质瘤标本131例，另取颅脑损伤内减压术中切除的正常脑组织标本32例作为对照。应用甲基化特异PCR及RT-PCR方法检测SOX7基因的甲基化状态及mRNA表达水平。采用Kplan-Meier法分析生存曲线。结果 胶质瘤SOX7基因甲基化率（71.8%，94/131）明显高于正常脑组织（32.4%，11/34；P<0.05）。高级别胶质瘤SOX7甲基化率（81.01%，64/79）明显高于低级别胶质瘤（57.69%，30/52）。胶质瘤SOX7 mRNA水平明显低于正常脑组织（P<0.05）。SOX7基因甲基化组生存期较非甲基化组明显缩短（P<0.01）。结论 胶质瘤SOX7基因甲基化率升高，SOX7在胶质瘤中表达水平下调，SOX7基因的甲基化状态与病人生存期密切相关。

Methylation state of SOX9 in human glioma and its clinical meanings

Objective To detect the methylation status and expression level of SOX7 in the human glioma and normal brain tissues, and evaluate their relationship with the tumorigenesis and prognosis in the patients with gliomas. Methods The methylation states and expression levels of SOX7 were determined respectively by methylation specific PCR and RT-PCR in 131 samples of gliomas tissues and 34 samples of the normal brain tissues. The analysis of survival curve was performed by Kplan-Meier method. Results The methylation rate of SOX7 (71.8%, 94/131) was significantly higher in the glioma tissues than that 32.4% (11/34) in the normal brain tissues (P<0.01). The methylation rate of SOX7 (81.01%, 64/79) was significantly higher in the WHO grade Ⅲ~Ⅳ gliomas tissues than that (57.69%, 30/52) in the WHO grade Ⅰ~Ⅱ gliomas tissues. The expression level of SOX7 mRNA was (0.43±0.15) significantly higher in the normal brain tissues than that (0.34±0.17) in the glioma tissues (P<0.01). The level of SOX7 mRNA expression (0.38±0.18) was significantly higher in the WHO grade Ⅰ~Ⅱ gliomas tissues than that (0.31±0.66) in the WHO grade Ⅲ~Ⅳ gliomas tissues (P<0.01). The survival time was significantly shorter in the patients with methylation of SOX7 gliomas than that in the patients with non-methylation of SOX7 gliomas (P<0.05). Conclusions SOX7 is hypermethylated and its expression level was downregulated in human glioma tissues. The methylation status of SOX7 in the gliomas tissues was closely correlated with glioma patient’s survival time.