| HPLC-PDA法同时测定水源水中主要3种微囊藻毒素方法的研究 |
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| 引用本文： | 黄宏南,邱文倩,庄宛,华永有,柯加法,俞晓玲. HPLC-PDA法同时测定水源水中主要3种微囊藻毒素方法的研究[J]. 海峡预防医学杂志, 2010, 16(1): 1-3 |
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| 作者姓名： | 黄宏南 邱文倩 庄宛 华永有 柯加法 俞晓玲 |
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| 作者单位： | 福建省疾病预防控制中心,福州,350001;厦门出入境,检验检疫局,福建,361026;福建医科大学,福州,350004 |
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| 摘 要： | [目的]建立水中微囊藻毒素(MC)含量的高效液相色谱初筛、光谱定性、质谱确认分析方法。[方法]以Waters Atlantis dC18(3μ2.1×100 mm),色谱质谱柱为分析柱,流动相:20%甲醇溶液,Surveyor二极管阵列检测器(PDA)和Finnigan LCQ Deca XP MAX质谱仪为检测器。[结果]回归方程和相关系数为:MC-RR y=7.401998×1025 x+5.028972×102,r=0.998;MC-LR y=5.689824×105x+5.925834×102,r=0.9996;MC-YR y=4.816086×105x+6.145898×102,r=0.9994。平均回收率为=95.1%~105.0%,RSD为3.00%~4.99%。[结论]用HPLC-PDA法检测水源水中的微囊藻毒素方法简便,重现性好,灵敏度高,定性准确,定量精确。
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| 关 键 词： | 微囊藻毒素(MC) 高效液相色谱(HPLC) 二极管阵列检测器(PDA) 质谱仪(MS) |
Research on the Simultaneous Determination of Three Microcystins in Water by HPLC-PDA |
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| Affiliation: | HUANG Hong-Nan,QIU Wen-Qian,ZHUANG Wan,et al(Fujian Center for Disease Control and Prevention,Fuzhou 350001,China) |
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| Abstract: | [Objective] To establish the determination method of MC-RR,MC-LR and MC-YR in water by HPLC-PDA and confirm by LC-MS.[Methods] With Waters Atlantis dC18 3 μ2.1×100mm as Chromatographic Column,mobile phase of 20% methanol,MC was detected by PDA and Finnigan LCQ Deca XP MAX MS detector.[Results] The linear regression function and correlation coefficient of 3 kinds of MC were as follows respectively： y=7.4019983×105x＋5.028972×102,r=0.998（MC-RR）;y=5.689824×105x＋5.925834×102,r=0.9996（MC-LR）;y=4.816086×105x＋6.145898×102,r=0.9994（MC-YR）.The average recovery rates =95.1%―105.0%,RSD =3.00%―4.99%.[Conclusion] The HPLC-PDA method is sample,sensitive,reliable and accurate for detecting of MC in water. |
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| Keywords: | Microcystins（MC-RR MC-LR MC-YR） High Performance Liquid Chromatography（HPLC） Photo Diode Array（PDA） Mass-spectrometer（MS） |
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