| 毒害艾美耳球虫江苏分离株抗原基因NA4的克隆与序列分析 |
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| 引用本文： | 张娜,严若峰,徐立新,李祥瑞.毒害艾美耳球虫江苏分离株抗原基因NA4的克隆与序列分析[J].寄生虫与医学昆虫学报,2008,15(4):193-197. |
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| 作者姓名： | 张娜 严若峰 徐立新 李祥瑞 |
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| 作者单位： | 南京农业大学动物医学院,南京,210095 |
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| 基金项目： | 国家"863"计划资助项目
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| 摘 要： | 采用直肠接种毒害艾美耳球虫（Eimeria necatrix）裂殖子方法对毒害艾美耳球虫卵囊进行纯化,用柔嫩艾美耳球虫子孢子表面抗原TA4基因特异性引物,以纯化E.necatrix孢子化卵囊总RNA为模板,RT-PCR方法克隆出了一段新基因序列。该基因全长747bp,共编码248个氨基酸,与国外Brothers等（1991）从E.necatrix孢子化卵囊表面得到的NA4基因同源性达99.7%,与柔嫩艾美耳球虫TA4（No.AJ586531.2）基因同源性达到91.4%,拥有与柔嫩艾美耳球虫TA4抗原基因相同序列的一段信号肽。从结果可得出,新克隆的基因为E.necatrix NA4基因。此基因已登录GenBank,登录号为EU523548。
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| 关 键 词： | 毒害艾美耳球虫 NA4基因 TA4基因 孢子化卵囊 |
CLONING AND SEQUENCE ANALYSIS OF NA4 GENE OF EIMERIA NECATRIX ISOLATED FROM JIANGSU PROVINCE IN CHINA |
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| ZHANG Na,YAN Ruo-Feng,XU Li-Xin,LI Xiang-Rui.CLONING AND SEQUENCE ANALYSIS OF NA4 GENE OF EIMERIA NECATRIX ISOLATED FROM JIANGSU PROVINCE IN CHINA[J].Acta Parasitologica et Medica Entomologica Sinica,2008,15(4):193-197. |
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| Authors: | ZHANG Na YAN Ruo-Feng XU Li-Xin LI Xiang-Rui |
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| Institution: | ZHANG Na YAN Ruo-Feng XU Li-Xin LI Xiang-Rui(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | Using specific primers of TA4 gene of E.tenella,a new gene was cloned with RT-PCR from the sporulated oocysts of E.necatrix isolated from Jiangsu province of China.The sequence analysis showed that the new gene was 747 bp in size and encoded 248 amino acids.Comparisons of the sequence of this gene with the reported NA4(Brothers et al.,1991)gene of E.necatrix and TA4(No.AJ586531.2)gene of E.tenella indicated that this new gene had nucleotide sequence homologies with the NA4 gene(99.4%)of E.necatrix and TA4 g... |
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| Keywords: | Eimeria necatrix Eimeria tenella NA4 gene TA4 gene Sporulated oocyst |
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