血管内皮生长因子C及其受体与宫颈癌临床和预后的关系

目的:观察血管内皮生长因子C(VEGF-C)与血管内皮生长因子受体3(VEGFR-3)在宫颈癌组织中的表达并分析其与临床及预后的关系。方法:免疫组织化学SP法检测80例宫颈癌组织中VEGF-C与VEGFR-3的表达,以30例正常宫颈组织为对照组。结果:VEGF-C在癌组织中呈高表达,癌周组织表达相对较低,但两组表达均高于对照组(P<0.05);癌组织中VEGF-C的表达与肿瘤的临床分期相关,淋巴结转移组VEGF-C的阳性表达率为69.6%明显高于无淋巴结转移组的49.1%,差异有统计学意义(P<0.05)。VEGFR-3在癌周组织表达较高,癌组织中表达较低,但都明显高于对照组(P<0.05);淋巴结转移组中的VEGFR-3在癌周组织标记的淋巴管密度为19.67±3.72明显高于无淋巴结转移组10.78±2.65,差异有统计学意义(P<0.05);Ⅰb期组VEGFR-3的表达15.78±5.67明显高于Ⅰa2期9.87±1.95,低于Ⅱa期18.78±4.32,随着宫颈癌临床分期及病理组织学分级的提高,VEGFR-3表达也随之增强,差异有统计学意义(P<0.05)。结论:VEGF-C和VEGFR-3与宫颈癌的发生发展密切相关,癌组织VEGF-C和癌周组织VEGFR-3表达水平的检测可能有利于临床评估宫颈癌的预后。

The relationship between the VEGF-C, VEGFR-3 and the clinical feature of cervical carcinoma

Objective:To observe the expression of VEGF-C and VEGFR-3 in the cervical carcinoma,then to analyze the relation of them with the clinical feature and prognosis.Methods:The VEGF-C and VEGFR-3 in the 80 cervical carcinoma were detected with immunohistochemical SP,and 30 cases of normal tissue was regarded as the control group.Results:The expression of VEGF-C in cervical carcinoma was higher than that of in intratumoral tissue,but both the two groups were higher than the control group.The difference was significant(P<0.05,P<0.05).The expression of VEGF-C in cervical carcinoma was related to the clinical stages.The positive rate of VEGF-C with lymphatic metastasis was 69.6%,which was much more than that(49.1%) of without lymphatic metastasis.The difference was significant(P<0.05).The expression of VEGFR-3 in intratumoral tissue was clear higher than that of in cervical carcinoma;both the two groups were higher than the normal group.The LVD stained by VEGFR-3 of intratumoral tissue with lymphatic metastasis was 19.67±3.72/case,which was more than that of without lymphatic metastasis(10.78±2.65/case),the difference was significant.The LVD of Ⅰb was more than that of Ⅰa2,but less than that of Ⅱa.With the increase of clinical stage and pathological class,the VEGFR-3 was enhanced.The difference was significant.Conclusion:VEGF-C and VEGFR-3 are related to the occurrence and development of cervical carcinoma.The level test of VEGF-C and VEGFR-3 is beneficial to estimate the prognosis of cervical carcinoma in clinical.