| 快速薄层扫描法测定苜蓿中总皂苷含量 |
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| 引用本文： | 许舒雯,龚祝南,丛晓东,张锋伦.快速薄层扫描法测定苜蓿中总皂苷含量[J].中国生化药物杂志,2006,27(4):235-236. |
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| 作者姓名： | 许舒雯 龚祝南 丛晓东 张锋伦 |
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| 作者单位： | 南京师范大学,生命科学学院,江苏,南京,210097 |
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| 摘 要： | 采用薄层扫描法测定苜蓿全草中的有效成分苜蓿皂苷的含量。方法以甲醇提取,硅胶G薄层板,正丁醇-乙酸乙酯-水(4:1:5)为展开剂,扫描测定,测定波长为450 nm,参比波长为540 nm。结果平均回收率为94.4%(n=4),RSD=3.1%。结论利用薄层扫描可直接测定苜蓿皂苷含量,该方法简便,重现性较好。
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| 关 键 词： | 苜蓿 皂苷 薄层扫描 |
| 文章编号： | 1005-1678（2006）04-0235-02 |
| 收稿时间： | 2006-03-15 |
| 修稿时间： | 2006-04-12 |
Determination of total triterpene saponin in Medicago sativa L. by TLC scanning |
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| XU Shu-wen,GONG Zhu-nan,CONG Xiao-dong,ZHANG Feng-lun.Determination of total triterpene saponin in Medicago sativa L. by TLC scanning[J].Chinese Journal of Biochemical Pharmaceutics,2006,27(4):235-236. |
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| Authors: | XU Shu-wen GONG Zhu-nan CONG Xiao-dong ZHANG Feng-lun |
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| Institution: | School of Life Scienec, Nanjing Normal University, Nanjing 210097, China |
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| Abstract: | PurposeAlfalfa saponin M of Medicago sativa L.was determined by the TLC scanning method.Methodsn-BuOH-ethylacetate-water(4∶1∶5)as the mobile phase,wavelength at λ_s=450 nm,and λ_R=540 nm.ResultsThe average recovery and RSD were 94.4%(n=4)and 3.1%,respectively.ConclusionThis method is convenient and reproducible. |
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| Keywords: | Alfalfa saponin TLC scanning |
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