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| 64DP IN THE NUCLEUS OF HUMAN HEPATOCYTE | |
| 引用本文： | 於利敏,姜众,童坦君. 64DP IN THE NUCLEUS OF HUMAN HEPATOCYTE[J]. 中国癌症研究, 1991, 3(3): 1-5. DOI: 10.1007/BF02955260 |
| 作者姓名： | 於利敏 姜众 童坦君 |
| 作者单位： | Yu Limin Jiang Zhong Tong TanjunDepartment of Biochemistry，Beijing Medical University Department of Pathology，Beijing Medical University |
| 摘 要： | It is reported that 64DP is a tumor-related human serum DNA binding protein. The significance of its existence in serum and the mechanism of its level changing during malignant and other diseases remain unknown. 64DP in hepatocellular carcinoma (HCC), hepatitis B, and normal livers was studied with immuno- histochemistry technique (PAP). While 12/20 HCC were positive for 64DP in the cytoplasm, the positive staining were only showed in the nuclei of most of the hepatitis B and normal livers. Auto-radiography of immobilized human chromatin with 125I-antibody against 64DP proved the existence of 64DP in chromatin. Incorporation of 3H- TdR in cultivated mouse fibroblasts showed that 64DP could inhibit cellular DNA synthesis with the inhibition rate over 30%. These results suggested that 64DP might be an inhibitory DNA binding protein, playing its physiological function in the nucleus. |
64DP in the nucleus of human hepatocyte |
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| Limin Yu,Zhong Jiang,Tanjun Tong. 64DP in the nucleus of human hepatocyte[J]. Chinese Journal of Cancer Research, 1991, 3(3): 1-5. DOI: 10.1007/BF02955260 | |
| Authors: | Limin Yu Zhong Jiang Tanjun Tong |
| Affiliation: | Yu Limin Jiang Zhong Tong TanjunDepartment of Biochemistry,Beijing Medical University Department of Pathology,Beijing Medical University |
| Abstract: | It is reported that 64DP is a tumor-related human serum DNA binding protein. The significance of its existence in serum and the mechanism of its level changing during malignant and other diseases remain unknown. 64DP in hepatocellular carcinoma (HCC), hepatitis B, and normal livers was studied with immuno- histochemistry technique (PAP). While 12/20 HCC were positive for 64DP in the cytoplasm, the positive staining were only showed in the nuclei of most of the hepatitis B and normal livers. Auto-radiography of immobilized human chromatin with 125I-antibody against 64DP proved the existence of 64DP in chromatin. Incorporation of 3H- TdR in cultivated mouse fibroblasts showed that 64DP could inhibit cellular DNA synthesis with the inhibition rate over 30%. These results suggested that 64DP might be an inhibitory DNA binding protein, playing its physiological function in the nucleus. |
| Keywords: | 64DP immunohistochemistry hepatocellular. |
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