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| 原代SD大鼠海马神经元扫描电镜形态学特点 | |
| 引用本文： | 蔡志平,李朝晖,石素琴,崔慧先,霍东升,石岩,贾建新. 原代SD大鼠海马神经元扫描电镜形态学特点[J]. 包头医学院学报, 2012, 28(1): 14-16 |
| 作者姓名： | 蔡志平 李朝晖 石素琴 崔慧先 霍东升 石岩 贾建新 |
| 作者单位： | 1. 河北医科大学人体解剖学教研室,河北石家庄050017;包头医学院人体解剖学教研室 2. 河北医科大学人体解剖学教研室,河北石家庄050017;河北医科大学第二医院神经外一科 3. 包头医学院第二附属医院药剂科 4. 河北医科大学人体解剖学教研室,河北石家庄,050017 5. 包头医学院人体解剖学教研室 |
| 摘 要： | 目的:观察体外培养的原代海马神经元扫描电镜形态学特点。方法:体外培养新生SD乳鼠原代海马神经元,行免疫细胞化学鉴定,用扫描电镜观察其形态学特点。结果:培养的海马神经元形态多样,梭形神经元胞体较大,伸出两个突起;神经元细胞有的基底宽广,突起有粗有细,与周围的神经元细胞连结紧密;锥体细胞从胞体发出2～3个突起,从粗大的突起上又分出突起;培养的神经元以锥体形为主,其次为梭形、三角形。结论:培养的神经元为MAP-2染色阳性,扫描电镜可见神经元胞体及突起形成神经元的特有结构。 |
| 关 键 词： | 海马神经元 原代培养 形态学 扫描电镜 |
Characteristics of SD Rat Hippocampal Neuron by Scanning Electron Microscopy |
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| Affiliation: | CAI Zhiping1,2,LI Zhaohui1,Shi Suqin2,et al(1.Human Anatomy Department,Hebei Medical University,Shijiazhuang 050017,China; 2.Human Anatomy Department,Baotou Medical College) |
| Abstract: | Objective: To observe morphological characteristics of primary SD rat hippocampal neuron with scanning electron microscopy.Methods: New born SD puppies was cultivated,with immunohistochemistry identification,and its morphological characteristics were observed with scanning electron microscopy.Results: Pimary cultivated neurons showed different shapes.Neuron body is larger,with 2 processes form it.Some cell bodies have broad bases,with p either thin processes or thick ones,and have a tight connection with around cell.Pyramid cells have 2-3 processes from cell bodies,with branches from those thicker ones.Cultivated cell mostly are pyramid cells,then spindle-shaped ones and triangle ones.Conclusion: Neurons are MAP-2 positive and scanning electron microscopy shows that cell body and process have special characteristics of neuron. |
| Keywords: | Hippocampal neuron Primary cultivation Morphology Scanning electron microscopy |
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