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人结肠癌HCTv2000细胞系耐药机制的初步研究
引用本文:张晓红,诸亚君,籍秀娟,李学汤. 人结肠癌HCTv2000细胞系耐药机制的初步研究[J]. 肿瘤学杂志, 1996, 0(1)
作者姓名:张晓红  诸亚君  籍秀娟  李学汤
作者单位:中国医学科学院肿瘤研究所!北京,100021,中国医学科学院肿瘤研究所!北京,100021,中国医学科学院药物研究所!北京,100050,中国医学科学院肿瘤研究所!北京,100021
基金项目:本课题为国家“八五”科技攻关项目
摘    要:HCTv2000是人结肠癌HCT-8细胞系经长春新碱选择而建立的耐药系。该系细胞对抗P-糖蛋白单抗呈阳性反应。编码P-精蛋白的多向耐药基因mdrl未见扩增或重排,但mdrlmRNA过度表达。异博定使细胞内3H-长春新碱蓄积增多,可部分逆转耐药。据此认为,mdrl基因的转录性激活是HCTv2000细胞耐药的重要原因,但有其他机制参与。

关 键 词:结肠癌  细胞系  耐药  mdrl基因  P-糖蛋白

Preliminary Study on Mechanism of Drug Resistance in HumanColon Cancer Cell Line HCTv2000
Zhang Xiaohong,Zhu Yajun, Ji Xiujuan,et al.. Preliminary Study on Mechanism of Drug Resistance in HumanColon Cancer Cell Line HCTv2000[J]. Journal of Chinese Oncology, 1996, 0(1)
Authors:Zhang Xiaohong  Zhu Yajun   Ji Xiujuan  et al.
Affiliation:Zhang Xiaohong,Zhu Yajun, Ji Xiujuan, et al.
Abstract:The HCTv2000 cells were selected with increasing concentrations of vincristine from a human coloncancer line (HCT-8). In the present study, the mechanism of drug resisbocepf HCTv2000 cells reintive to the mdrl gene was observed. Immunocytochemical staining showed a poaitive response to monoclonal antibodies (JSB-1and MRK-16) against P-glycoptotein (P-GP) encoded by the mdrl gene. A high level of mdrl mRNA was found bymeans of Slot blotting and Northern blotting. However, no evidence of mdrl gene arnplification or rearrangementwas detectal. Verapamil increased the accumulation of 3H-vincristine indse the cells but did not appear to reverse drug resistance to vincrithee completely, demonstrating thet there ndght be other mechanism(8) also involvedin the HCTv2000 cells.
Keywords:Colon cancer Cell line Drug resistance Mdrl gene P-glycoprotein
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