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| 佛波酯诱导的蛋白激酶C活化对扭转蛋白A亚细胞分布的影响 | |
| 引用本文： | 胡小辉,郑珍珍,盛丽琴,潘平雷,宋伟,黄睿,商慧芳. 佛波酯诱导的蛋白激酶C活化对扭转蛋白A亚细胞分布的影响[J]. 华西医学, 2011, 0(3): 321-324 |
| 作者姓名： | 胡小辉 郑珍珍 盛丽琴 潘平雷 宋伟 黄睿 商慧芳 |
| 作者单位： | 四川大学华西医院神经内科; |
| 基金项目： | 国家自然科学基金资助项目(30400144)~~ |
| 摘 要： | 目的 探讨佛波酯激活的蛋白激酶C与扭转蛋白A在亚细胞成分中的表达之间的关系.方法 采用免疫荧光法观察扭转蛋白A在原代培养的神经元和小鼠胚胎成纤维细胞(NIH 3T3细胞)中的分布.运用蛋白质印迹法分析蛋门激酶C和扭转蛋白A在细亚细胞成分中的表达.结果 扭转蛋白A在NIH 3T3细胞中的表达类似于神经元.扭转蛋白A在细胞... |
| 关 键 词： | 佛波酯 蛋白激酶C 扭转蛋白A 肌张力障碍 |
Effect of Phorbol 12-Myristate 13-Acetate Induced Protein Kinase C Activation on the TorsinA Subcellular Distribution |
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| HU Xiao-hui,ZHENG Zhen-zhen,SHENG Li-qin,PAN Ping-lei,SONG Wei,HUANG Rui,SHANG Hui-fang. Effect of Phorbol 12-Myristate 13-Acetate Induced Protein Kinase C Activation on the TorsinA Subcellular Distribution[J]. West China Medical Journal, 2011, 0(3): 321-324 | |
| Authors: | HU Xiao-hui ZHENG Zhen-zhen SHENG Li-qin PAN Ping-lei SONG Wei HUANG Rui SHANG Hui-fang |
| Affiliation: | HU Xiao-hui,ZHENG Zhen-zhen,SHENG Li-qin,PAN Ping-lei,SONG Wei,HUANG Rui,SHANG Hui-fang.Department of Neurology,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,P.R.China |
| Abstract: | Objective To investigate the relationship between the phorbol 12-myristate 13-acetate(PMA) activated protein kinase C(PKC) and the subcellular expression of TorsinA protein.Methods The expression of TorsinA in the primary cultured neurons and the NIH 3T3 cells was detected by immunofluorescence.The expression of PKC and TorsinA in subcellular fraction was analyzed by the western blotting.Results The expression pattern of TorsinA in NIH 3T3 cells was similar to neuron.PMA,an activator of PKC,did not promote ... |
| Keywords: | Phorbol 12-myristate 13-acetate Protein kinase C TorsinA Dystonia |
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