荧光抗体法及厌氧菌培养法检出类杆菌与梭状杆菌的质量控制

本文为1983年1月至1984年7月374例次临床标本的脆弱类杆菌、产黑类杆菌与核粒梭状杆菌的荧光抗体法(IFA)与厌氧菌培养法的对比,并对二法的质量控制如符合率、敏感性、特异性、预示值、抗血清的保存期限作了比较。说明二法符合率良好(88.5～95.99％)。IFA有快速诊断的优点外,并有下列的特点: 1.IFA可检查厌氧菌培养法的质量,经对比,证明我们现用厌氧菌培养法宜于分离产黑类杆菌、核粒梭状杆菌,不宜于分离脆弱类杆菌,对后一种厌氧菌培养法有待改进。 2.国外已有压氧菌专用荧光抗体试剂出售,国内并无此项专用试剂,但可自制厌氧菌抗血清,向北京或上海生物制品研究所购买羊抗兔免疫球蛋白荧光抗体,可作IFA的间接法快速诊断之用. 3.经三年来(1982～1983年102例次,1983～1984年的374例次)三种厌氧菌的IFA与厌氧菌培养法对比,都证明IFA可提高阳性检出率。 并对二法存在的问题略加讨论。

The Quality Control of Immunofluorescent Antibody Test and Anaerobic Culture in Study of Bacteroides and Fusobacterium

A comparative study between the indirect immunofluorescent antibody test and anaerobic culture in the study of Bacteroides fragilis, Bacteroides melani-nogenicus, and Fusobacterium nucleatum in 374 clinical specimens was carried out. The data concerning the quality control of the two tests including the rate of correlation, the difference of the sensitivity and specificity,the predictive values, and the time of antiserum preservation were analyzed. It was found that immunofluorescent antibody test had a higher rate of detection than anaerobic culture. It can be cartain that if immunofluorescent antibody test is negtive, it is more likely that the anaerobic bacteria are abscent. The rate of correlation between the two tests was 88.5~95.99%. In addition, immunofluorescent antibody test can be used to verify the quality of anaerobic culture. It was found that our routine culture for B, fragilis was inperfect and waited for improvement.